In Vitro Probiotic Potential and Safety Evaluation (Hemolytic, Cytotoxic Activity) of Bifidobacterium Strains Isolated from Raw Camel Milk

Microorganisms

卷:8

Saima Naz¹  Saima Tehseen²  Rabia Iqbal²  Iqra Yasmin²  Atif Liaqat³  Samreen Ahsan³  MuhammadFarhan Jahangir Chughtai³  Tariq Mehmood³  Adnan Khaliq³  WahabAli Khan⁴  Muhammad Saeed⁴  Saira Tanweer⁵ 

[1] Department of Clinical Nutrition, Nur International University, Lahore 54000, Pakistan;

[2] Department of Food Science and Technology, Government College Women University, Faisalabad 38040, Pakistan;

[3] Department of Food Science and Technology, Khwaja Fareed University of Engineering and Information Technology, Rahem Yar Khan 64200, Pakistan;

[4] National Institute of Food Science and Technology, Faculty of Food, Nutrition and Home Sciences, University of Agriculture, Faisalabad 38040, Pakistan;

[5] University College of Agriculture and Environmental Sciences, Islamia University, Bahawalpur 63100, Pakistan;

关键词: probiotic potential;    antimicrobial activity;    camel milk: b-11;    pathogens;   

DOI  :  10.3390/microorganisms8030354

来源: DOAJ

【 摘 要 】

The present study was designed to isolate Bifidobacterium strains from raw camel milk and to investigate their probiotic characteristics. Among 35 isolates, 8 were identified as Gram-positive, catalase negative, non-spore forming, non-motile and V or Y shaped rods. B-2, B-5, B-11, B-19 and B-28 exhibited good survival at low pH and high bile salt concentration. Most of the isolates were resistant to nalidixic acid, fusidic acid, polymyxin B, neomycin, streptomycin, gentamicin, rifampicin and kanamycin. Furthermore, the production of exopolysaccharides (EPS), adhesion characteristics, antioxidant properties, antagonistic activities, nitrite reduction and cholesterol assimilation were also studied. Isolate B-11 was chosen because it exhibited most of the probiotic properties among all the tested isolates. It is identified as the member of Bifidobacterium longum group through 16S rRNA gene sequencing and named as B. longum B-11. B. longum B-11 was further selected for in vivo attachment to rat intestine and scanning electron micrographs revealed that attachment of a large number of rods shaped bacterial cell. Our findings suggest that B. longum B-11 processes excellent attributes to be used as potential probiotic in the development of functional probiotic food.

【 授权许可】

Unknown