Glycan analysis of colorectal cancer samples reveals stage-dependent changes in CEA glycosylation patterns

Clinical Proteomics

卷:15

Shaojie Yang¹  Tiancheng Zhan²  Dengbo Ji²  Yaming Liu³  Qianqian Li³  Qianqian Zhao³  Zaian Deng³  Yan Li³ 

[1]GuangDong Bio-healtech Advanced Co., Ltd.

[2]|Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Colorectal Surgery, Peking University Cancer Hospital and Institute

[3]|Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences

关键词: Colorectal cancer;    Carcinoembryonic antigen;    Glycosylation;    Lectin microarray;   

DOI  :  10.1186/s12014-018-9182-4

来源: DOAJ

【 摘 要 】

Abstract Background Carcinoembryonic antigen (CEA) is a glycoprotein associated with colorectal cancer (CRC). While the functions of its gene and protein have been fully characterized, its post-translational modifications in the context of CRC development remain undefined. Methods To show the correlation between the different stages of CRC development and changes in the glycosylation patterns of CEA, we analyzed CEA in tumor tissues (CEA-T) and paired tumor-adjacent normal tissues (CEA-A) from 53 colorectal cancer patients using a high-density lectin microarray containing 56 plant lectins. Results We detected higher expression levels of fucose, mannose and Thomsen–Friedenreich antigen, and lower expression levels of N-acetylgalactosamine, N-acetylglucosamine, galactose, branched and bisecting N-glycans on CEA in the tumor tissues relative to the tumor-adjacent normal tissues. Furthermore, a combinatorial assessment of 9 lectins is sufficient to distinguish CRC tumor tissues from tumor-adjacent normal tissues with 83% sensitivity and ~ 90% specificity. Moreover, the levels of N-acetylgalactosamine, mannose, galactose, N-acetylglucosamine on CEA showed a downward trend after first experiencing an increase at Stage II with the stages of CRC. Conclusions Our insights into the changing CEA glycosylation patterns and their role in the development of CRC highlight the importance of glycan variants on CEA for early clinical detection and staging of CRC.

【 授权许可】

Unknown