An Assessment of Cell Culture Plate Surface Chemistry for in Vitro Studies of Tissue Engineering Scaffolds

Journal of Functional Biomaterials

卷:6

Nikola Ristovski¹  Maria A. Woodruff¹  Elena García-Gareta¹  Keith A. Blackwood¹  Christina Theodoropoulos¹  Alexander Röder¹ 

[1] Institute of Health & Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, Queensland, 4059, Australia;

关键词: tissue engineering;    3D scaffolds;    cell attachment;    poly-ε-caprolactone;   

DOI  :  10.3390/jfb6041054

来源: DOAJ

【 摘 要 】

The use of biopolymers as a three dimensional (3D) support structure for cell growth is a leading tissue engineering approach in regenerative medicine. Achieving consistent cell seeding and uniform cell distribution throughout 3D scaffold culture in vitro is an ongoing challenge. Traditionally, 3D scaffolds are cultured within tissue culture plates to enable reproducible cell seeding and ease of culture media change. In this study, we compared two different well-plates with different surface properties to assess whether seeding efficiencies and cell growth on 3D scaffolds were affected. Cell attachment and growth of murine calvarial osteoblast (MC3T3-E1) cells within a melt-electrospun poly-ε-caprolactone scaffold were assessed when cultured in either “low-adhesive” non-treated or corona discharged-treated well-plates. Increased cell adhesion was observed on the scaffold placed in the surface treated culture plates compared to the scaffold in the non-treated plates 24 h after seeding, although it was not significant. However, higher cell metabolic activity was observed on the bases of all well-plates than on the scaffold, except for day 21, well metabolic activity was higher in the scaffold contained in non-treated plate than the base. These results indicate that there is no advantage in using non-treated plates to improve initial cell seeding in 3D polymeric tissue engineering scaffolds, however non-treated plates may provide an improved metabolic environment for long-term studies.

【 授权许可】

Unknown