Dicalcium Phosphate Coated with Graphene Synergistically Increases Osteogenic Differentiation In Vitro

Coatings

卷:8

Jae Min Cha¹  Seung Jo Jeong¹  Su-Jin Song²  Young-Jun Lim²  Dong-Wook Han³  Jun Jae Lee³  Bongju Kim⁴  Suck Won Hong⁵  Yong Cheol Shin⁶ 

[1] Nanotechnology, Pusan National University, Busan 46241, Korea;

[2] Department of Cogno-Mechatronics Engineering, College of Nanoscience &

[3] Department of Prosthodontics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Korea;

[4] GS Medical Co., Ltd., Cheongju-si, Chungcheongbuk-do 28161, Korea;

[5] Medical Device Research Center, Samsung Medical Center, Seoul 06351, Korea;

[6] Research Center for Energy Convergence Technology, Pusan National University, Busan 46241, Korea;

关键词: reduced graphene oxide;    dicalcium phosphate;    hybrid composite;    MC3T3-E1 preosteoblast;    osteogenic differentiation;    bone tissue regeneration;   

DOI  :  10.3390/coatings8010013

来源: DOAJ

【 摘 要 】

In recent years, graphene and its derivatives have attracted much interest in various fields, including biomedical applications. In particular, increasing attention has been paid to the effects of reduced graphene oxide (rGO) on cellular behaviors. On the other hand, dicalcium phosphate (DCP) has been widely used in dental and pharmaceutical fields. In this study, DCP composites coated with rGO (DCP-rGO composites) were prepared at various concentration ratios (DCP to rGO concentration ratios of 5:2.5, 5:5, and 5:10 μg/mL, respectively), and their physicochemical properties were characterized. In addition, the effects of DCP-rGO hybrid composites on MC3T3-E1 preosteoblasts were investigated. It was found that the DCP-rGO composites had an irregular granule-like structure with a diameter in the range order of the micrometer, and were found to be partially covered and interconnected with a network of rGO. The zeta potential analysis showed that although both DCP microparticles and rGO sheets had negative surface charge, the DCP-rGO composites could be successfully formed by the unique structural properties of rGO. In addition, it was demonstrated that the DCP-rGO composites significantly increased alkaline phosphatase activity and extracellular calcium deposition, indicating that the DCP-rGO hybrid composites can accelerate the osteogenic differentiation by the synergistic effects of rGO and DCP. Therefore, in conclusion, it is suggested that the DCP-rGO hybrid composites can be potent factors in accelerating the bone tissue regeneration.

【 授权许可】

Unknown