Nano polyamidoamine-G7 dendrimer synthesis and assessment the antibacterial effect in vitro

Tehran University Medical Journal

卷:74

MahdiFarzadkia¹  MitraGholami¹  Soudabeh Alizadeh Matboo²  ShahramNazari³  Seyed MohsenMohseni⁴  FakhraddinAkbari Dourbash⁵  MeysamHasannejad⁶ 

[1] Department of Environmental Health Engineering, Iran University of Medical Sciences, Tehran, Iran.;

[2] Department of Environmental Health Engineering, School of Public Health, Ardabil University of Medical Sciences, Ardabil, Iran.;

[3] Department of Environmental Health Engineering, School of Public Health, Member of Student Research Committee, Iran University of Medical Sciences, Tehran, Iran.;

[4] Department of Environmental Health Engineering, School of Public Health, Qom University of Medical Sciences, Qom, Iran.;

[5] Department of Materials Science and Engineering, Tarbiat Modares University, Tehran, Iran.;

[6] Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.;

关键词: anti-bacterial agents;    microbial sensitivity tests;    PAMAM;    polyamidoamine;    dendrimers;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: Nano scale dendrimers are macromolecules synthetic which frequently used in medical and health field. Because traditional antibiotics inevitably induce bacterial resistance, which is responsible for many treatment failures, there is an urgent need to develop novel antibiotic drugs. This study was aimed to examine Synthesis and the antibacterial effect of NanoPolyamidoamine-G7 (NPAMAM-G7) dendrimer on Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus.Methods: In this experimental study that has been conducted in June 2015 in the Laboratory of Microbiology, Iran University of Medical Science, NPAMAM-G7 dendrimers was synthesized by Tomalia’s divergent growth approach. The antibacterial effects of NPAMAM-G7 dendrimer were studied by disc diffusion and micro-dilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against gram-positive and gram-negative bacteria were determined according to Clinical and Laboratory Standards Institute (CLSI) guideline. Standard discs were prepared using different concentrations of dendrimer on Mueller-Hinton agar plates.Results: Zone of inhibition in concentration 25 μg/ml of NPAMAM-G7 dendrimers for Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus were 26, 38, 36, 22 and 25 mm, respectively. Regarding the zone of inhibition in gram negative bacteria with gram positive ones was P= 0.16 and was not significant difference. The MIC for Salmonella Typhi was 0.025, for Proteus Mirabilis, Bacillus Subtilis, Staphylococcus Aureus and Escherichia Coli was 0.25 μg/ml. The MBC for Salmonella Typhi was 25μg/ml, for Proteus Mirabilis and Bacillus Subtilis was 50 μg/ml and for Escherichia Coli and Staphylococcus Aureus was 100 μg/ml. The least of sensitivity against NPAMAM-G7 related to Escherichia Coli and Staphylococcus Aureus and the most of sensitivity related to Salmonella Typhi.Conclusion: The NPAMAM-G7 dendrimer with end amine groups exhibited a positive impact on the removal of standard strains, gram-positive and gram-negative bacteria. Therefore, it is possible to use these nanodendrimers as antibacterial in the future.

【 授权许可】

Unknown