Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount

Analytica

卷:2

Chihiro Kitaoka¹  Tatsuya Fujino²  Jiawei Xu²  Toshiki Horikoshi²  Yosuke Fujii³  Takashi Asano⁴ 

[1] Criminal Investigation Laboratory, Metropolitan Police Department, 2-1-1 Kasumigaseki, Chiyoda-ku, Tokyo 100-8929, Japan;

[2] Department of Applied Chemistry, Graduate School of Science and Engineering, Toyo University, 2100 Kujirai, Kawagoe 350-8585, Japan;

[3] Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0379, Japan;

[4] Forensic Science Laboratory, Metropolitan Police Department, 2-1-1 Kasumigaseki, Chiyoda-ku, Tokyo 100-8929, Japan;

关键词: matrix-assisted laser desorption ionization (MALDI);    surface-assisted laser desorption ionization (SALDI);    fingerprint;    acetaminophen;    zeolite;   

DOI  :  10.3390/analytica2030008

来源: DOAJ

【 摘 要 】

The ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the fingerprint taken 4 h after AAP ingestion, not only AAP but also the glucuronic acid conjugate of AAP (GAAP), caffeine (Caf), ethenzamide (Eth), salicylamide (Sala; a metabolite of Eth), and urea were detected. Fingerprints were collected over time to determine how the amounts of AAP and its metabolite changed with time, and the time dependence of the peak intensities of protonated AAP and GAAP was measured. It was found that the increase of [GAAP+H]⁺ peak started later than that of [AAP+H]⁺ peak, reflecting the metabolism of AAP. Both AAP and GAAP reached maximum concentrations approximately 3 h after ingestion, and were excreted from the body with a half-life of approximately 3.3 h. In addition, fingerprint preservation was confirmed by optical microscopy, and fingerprint shape was retained even after laser irradiation of the fingerprint. Our method may be used in fingerprint analysis.

【 授权许可】

Unknown