Molecular identification of Paragonimus ohirai and P. westermani from Anhui Province, China

Parasite

卷:7

关键词: Paragonimus ohirai;    Paragonimus westermani;    Anhui Province;    China;    internal transcribed spacer 2 (ITS2);   

DOI  :  10.1051/parasite/2000074305

来源: DOAJ

【 摘 要 】

Nucleotide sequences of the internal transcribed spacer 2 (ITS2) region were determined from seven adults of species Paragonimus collected from Jinde and Xiuning Counties, Anhui Province, China. Among these, the nucleotide sequence obtained from one Paragonimus adult (Jinde County) was identical to the ITS2 sequence of P. ohirai previously reported. In order to confirm the result, partial regions of mitochondrial cytochrome C oxidase (COI) and NADH dehydrogenase 1 (ND1) from the putative P. ohirai sample were further sequenced. They showed a high level of similarity with those of P. ohirai, COI (99.7 %) and ND1 (99.5 %), supporting the result obtained from the ITS2. In addition to this, we designed P. ohirai and P. westermani specific primers (BDW and BD2O H) from ITS2 to identify P. westermani and P. ohirai easily and rapidly. After testing utility of the primers, they were applied to identify seven unidentified Paragonimus samples collected from Jinde and Xiuning Counties, China. All the examined samples showed P. westermani band pattern, and it was reconfirmed by sequencing their ITS2 regions that they are P. westermani. This result indicates that the two newly designed specific primers could be quite helpful for easily identifying P. westermani and P. ohirai, that most of Paragonimus in Jinde and Xiuning Counties consist of P. westermani, and that P. ohirai exists in Jinde County with minority.

【 授权许可】

Unknown