Probiotic Characterization and Population Diversity Analysis of Gut-Associated Pediococcus acidilactici for Its Potential Use in the Dairy Industry

Applied Sciences

卷:11

Hafsa Kanwal¹  Fariha Masood Siddiqui¹  Freeha Zulfiqar¹  Amara Nawaz¹  Carla Sabia²  Muhammad Aqeel³  Shakira Ghazanfar³  Alessandro Di Cerbo⁴ 

[1] Department of Life Sciences, Abasyn University, Islamabad 44000, Pakistan;

[2] Department of Life Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy;

[3] National Agricultural Research Centre, National Institute of Genomics and Agriculture Biotechnology (NIGAB), Park Road, Islamabad 45500, Pakistan;

[4] School of Bioscience and Veterinary Medicine, University of Camerino, 62024 Matelica, Italy;

关键词: Pediococcus acidilactici;    16S rRNA;    probiotic;    population genetic analysis;    AMOVA;    MSN;   

DOI  :  10.3390/app11209586

来源: DOAJ

【 摘 要 】

In recent years, gut-tailored probiotics have been proven to be beneficial for host health. Probiotic strains such as lactic acid bacteria (LAB) are known to exhibit antimicrobial activity, acting as natural substitutes for the regulation of foodborne pathogens. In the present study, a complete analysis, isolation, biochemical characterization, and molecular identification of Pediococcus acidilactici (NMCC-11) from Nili Ravi water buffalo (Bubalis bubalis) gut was carried out. NMCC-11 showed the best enzymatic potential, antimicrobial activity against known pathogenic strains, and survivability at a wide pH range (pH 4–pH 6) out of all isolates. The isolates were screened for their antimicrobial activity against the five most infectious microbes such as Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC9027), Staphylococcus aureus (ATCC6538), Listeria monocytogenes (ATCC13932), and Bacillus cereus (ATCC 11778) using the agar-well diffusion method. Moreover, after NMCC-11 isolation, a comparative diversity analysis against a variety of other randomly selected strains from around the world was carried out using R software. This study showed relatively low genetic diversity, which also contributed to the claim of the stability of this probiotic strain and its potential use as a starter culture and feed probiotic in the dairy industry. However, further studies are certainly warranted to determine its optimal dosage, time frame, and intake frequency.

【 授权许可】

Unknown