Frontiers in Immunology | 卷:8 |
Butyrate Conditions Human Dendritic Cells to Prime Type 1 Regulatory T Cells via both Histone Deacetylase Inhibition and G Protein-Coupled Receptor 109A Signaling | |
Bart Everts1  Maria Yazdanbakhsh1  Leonard R. Pelgrom1  Alwin J. van der Ham1  Maria M. M. Kaisar2  | |
[1] Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, Netherlands; | |
[2] Faculty of Medicine, Department of Parasitology, Universitas Indonesia, Jakarta, Indonesia; | |
关键词: short-chain fatty acids; butyrate; dendritic cells; type 1 regulatory T cells; histone deacetylases; G-coupled protein receptor 109A; | |
DOI : 10.3389/fimmu.2017.01429 | |
来源: DOAJ |
【 摘 要 】
Recently, it has become clear that short-chain fatty acids (SCFAs), and in particular butyrate, have anti-inflammatory properties. Murine studies have shown that butyrate can promote regulatory T cells via the induction of tolerogenic dendritic cells (DCs). However, the effects of SCFAs on human DCs and how they affect their capacity to prime and polarize T-cell responses have not been addressed. Here, we report that butyrate suppresses LPS-induced maturation and metabolic reprogramming of human monocyte-derived DCs (moDCs) and conditions them to polarize naive CD4+ T cells toward IL-10-producing type 1 regulatory T cells (Tr1). This effect was dependent on induction of the retinoic acid-producing enzyme retinaldehyde dehydrogenase 1 in DCs. The induction of retinaldehyde dehydrogenase activity and Tr1 cell differentiation by butyrate was dependent on simultaneous inhibition of histone deacetylases and signaling through G protein-coupled receptor 109A. Taken together, we reveal that butyrate is a potent inducer of tolerogenic human DCs, thereby shedding new light on the cellular and molecular mechanisms through which SCFAs can exert their immunomodulatory effects in humans.
【 授权许可】
Unknown