Beet western yellows virus (BWYV): Aspect of Outbreak and Survey, and First Complete Genome Sequence of a Korea Isolate of BWYV

Research in Plant Disease

卷:24

Jeong-Sun Kim¹  Jae Sun Moon²  Seungmo Lim²  Jonghee Oh³  Hong Kyu Lee³  Su-Heon Lee³  Chung Youl Park³ 

[1] Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administraion, Wanju 55365, Korea;

[2] Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea;

[3] School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea;

关键词: Beet western yellows virus;    Large-scale oligonucleotide chip;    Paprika;   

DOI  :  10.5423/RPD.2018.24.4.276

来源: DOAJ

【 摘 要 】

In 2010, foliar symptoms were observed in the paprika leaves in Jinju city, Korea. Beet western yellows virus (BWYV) was identified in paprika by using the large-scale oligonucleotide chip assay. To investigate the occurrence of BWYV, a survey was performed on various crops, including paprika, from 2011 to 2014. Further, the presence of BWYV was consistently verified through literature survey from 2015 to 2017. BWYV infection has been identified in Solanaceae crops (bell pepper, hot pepper, and paprika), various weeds, and green peach aphids and it occurs on a nationwide scale. Cultivation using organic methods involved natural enemies and showed a high BWYV infection rate, which was more than that for conventional cultivation methods in greenhouse. The complete genome sequence of BWYV isolated from paprika was determined for the first time. The genome of the BWYV-Korea isolate consists of 5750 nucleotides and has six open reading frames. Sequence identity results showed maximum similarity between the BWYV-Korea isolate and the BWYV LS isolate (identity ＞ 90%). This study is the first report of BWYV infecting paprika in Korea. The survey revealed that BWYV is naturalized in the domestic ecology of Korea.

【 授权许可】

Unknown