Identification and Functional Analysis of lncRNA by CRISPR/Cas9 During the Cotton Response to Sap-Sucking Insect Infestation

Frontiers in Plant Science

卷:13

William D. Batchelor¹  Huaguo Zhu²  Daojun Yuan³  Jianying Li³  Jie Zhang³  Zhongping Xu³  Huan Si³  Qiongqiong Wang³  Jiawei Zou³  Fuhui Zhu³  Sumbul Saeed³  Qingying Meng³  Shuangxia Jin³  Muna Alariqi³  Xianlong Zhang³ 

[1] Biosystems Engineering, Auburn University, Auburn, AL, United States;

[2] College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, China;

[3] College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China;

[4] National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China;

关键词: cotton;    CRISPR/Cas9;    lncRNA;    RNA-Seq;    JA signaling;    plant-herbivore interaction;   

DOI  :  10.3389/fpls.2022.784511

来源: DOAJ

【 摘 要 】

Sap-sucking insects cause severe damage to cotton production. Long non-coding RNAs (lncRNAs) play vital regulatory roles in various development processes and stress response, however, the function of lncRNAs during sap-sucking insect infection in cotton is largely unknown. In this study, the transcriptome profiles between resistant (HR) and susceptible (ZS) cotton cultivars under whitefly infestation at different time points (0, 4, 12, 24, and 48 h) were compared. A total of 6,651 lncRNAs transcript and 606 differentially expressed lncRNAs were identified from the RNA-seq data. A co-expression network indicated that lncA07 and lncD09 were potential hub genes that play a regulatory role in cotton defense against aphid infestation. Furthermore, CRISPR/Cas9 knock-out mutant of lncD09 and lncA07 showed a decrease of jasmonic acid (JA) content, which potentially lead to increased susceptibility toward insect infestation. Differentially expressed genes between wild type and lncRNA knock-out plants are enriched in modulating development and resistance to stimulus. Additionally, some candidate genes such as Ghir_A01G022270, Ghir_D04G014430, and Ghir_A01G022270 are involved in the regulation of the JA-mediated signaling pathway. This result provides a novel insight of the lncRNA role in the cotton defense system against pests.

【 授权许可】

Unknown