The Protective Effects of Ciji-Hua’ai-Baosheng II Formula on Chemotherapy-Treated H22 Hepatocellular Carcinoma Mouse Model by Promoting Tumor Apoptosis

Frontiers in Pharmacology

卷:9

Yuewen Gong²  Jiaqi Yang²  Yangxinzi Xu³  Shuqiong Huang⁴  Biqian Fu⁴  Dawei Lu⁴  Yanan Wang⁴  Xiangyang Zhai⁴  Shengyan Xi⁵  Yanhui Wang⁵  Yingkun Qiu⁶  Jing Wang⁶ 

[1] Cancer Research Center, Xiamen University, Xiamen, China;

[2] College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada;

[3] Department of Physiology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada;

[4] Department of Traditional Chinese Medicine, Medical College, Xiamen University, Xiamen, China;

[5] Department of Traditional Chinese Medicine, Xiang’an Hospital of Xiamen University, Xiamen, China;

[6] School of Pharmaceutical Sciences, Xiamen University, Xiamen, China;

关键词: Ciji-Hua’ai-Baosheng II Formula;    chemotherapy;    H22 hepatocellular carcinoma;    Bcl-2;    Bax;    caspase-3;   

DOI  :  10.3389/fphar.2018.01539

来源: DOAJ

【 摘 要 】

Ciji-Hua’ai-Baosheng II Formula (CHB-II-F) is a traditional Chinese medical formula that has been shown in clinical practice to relieve side effects of chemotherapy and improve quality of life for cancer patients. In order to understand the mechanism of its protective effects on chemotherapy, mice with transplanted H22 hepatocellular carcinoma were employed in this study. Ninety-two mice were injected subcutaneously with H22 HCC cell suspension into the right anterior armpit. After mice were treated with 5-fluorine pyrimidine (5-FU), they were divided into six groups as untreated group, 5-FU group, 5-FU plus Yangzheng Xiaoji Capsule group and three groups of 5-FU plus different concentrations of CHB-II-F. Twenty mice were euthanized after 7 days of treatment in untreated and medium concentration of CHB-II-F groups and all other mice were euthanized after 14 days of treatment. Herbal components/metabolites were analyzed by UPLC-MS. Tumors were evaluated by weight and volume, morphology of light and electron microscope, and cell cycle. Apoptosis were examined by apoptotic proteins expression by western blot. Four major components/metabolites were identified from serum of mice treated with CHB-II-F and they are β-Sitosterol, Salvianolic acid, isobavachalcone, and bakuchiol. Treatment of CHB-II-F significantly increased body weights of mice and decreased tumor volume compared to untreated group. Moreover, CHB-II-F treatment increased tumor cells in G0-G1 transition instead of in S phase. Furthermore, CHB-II-F treatment increased the expression of pro-apoptotic proteins and decreased the expression anti-apoptotic protein. Therefore, CHB-II-F could improve mice general condition and reduce tumor cell malignancy. Moreover, CHB-II-F regulates apoptosis of tumor cells, which could contribute its protective effect on chemotherapy.

【 授权许可】

Unknown