Mutations in the Reverse Transcriptase and Protease Genes of Human Immunodeficiency Virus-1 from Antiretroviral Naïve and Treated Pediatric Patients

Viruses

卷:7

Dinesh Bure¹  Muzamil A. Makhdoomi¹  Somi Sankaran Prakash¹  Hilal A. Parray¹  Kalpana Luthra¹  Rajesh Kumar¹  Rakesh Lodha²  Ravinder Singh²  Sushil K. Kabra² 

[1] Department of Biochemistry, All India Institute of Medical Sciences, New Delhi 110029, India;

[2] Department of Pediatrics, New Delhi 110029, India;

关键词: drug resistance;    reverse transcriptase;    protease;    mutation;    nucleoside RT inhibitors;    non-nucleoside RT inhibitors;   

DOI  :  10.3390/v7020590

来源: DOAJ

【 摘 要 】

The success of highly active antiretroviral therapy (HAART) is challenged by the emergence of resistance-associated mutations in human immunodeficiency virus-1 (HIV-1). In this study, resistance associated mutations in the reverse transcriptase (RT) and protease (PR) genes in antiretroviral therapy (ART)  naïve and treated HIV-1 infected pediatric patients from North India were evaluated. Genotyping was successfully performed in 46 patients (30 ART naive and 16 treated) for the RT gene and in 53 patients (27 ART naive and 26 treated) for PR gene and mutations were identified using Stanford HIV Drug Resistance Database. A major drug resistant mutation in RT gene, L74I (NRTI), and two such mutations, K101E and G190A (NNRTI), were observed in two ART naïve patients, while M184V was detected in two ART treated patients. Overall, major resistance associated mutations in RT gene were observed in nine (30%) and seven (36%) of ART naïve and treated children respectively. Minor mutations were identified in PR gene in five children. Few non-clade C viral strains (≈30%) were detected, although subtype C was most predominant. The screening of ART naïve children for mutations in HIV-1 RT and protease genes, before and after initiation of ART is desirable for drug efficacy andgood prognosis.

【 授权许可】

Unknown