Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers

Toxins

卷:12

BrookeA. Clemmons¹  PhillipR. Myer¹  EmilyA. Melchior-Tiffany¹  JuanF. Cordero¹  TaylorB. Ault-Seay¹  KyleJ. McLean¹  GaryE. Bates²  JackP. Goodman³  Huihua Ji⁴  JamesL. Klotz⁵  MichaelD. Flythe⁵ 

[1] Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA;

[2] Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA;

[3] Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA;

[4] Kentucky Tobacco Research and Development Center, University of Kentucky, Lexington, KY 40546, USA;

[5] USDA-ARS, Forage-Animal Production Research Unit, Lexington, KY 40546, USA;

关键词: beef cattle;    endophyte;    ergot alkaloid;    fescue toxicosis;    isoflavone;    metabolites;   

DOI  :  10.3390/toxins12120744

来源: DOAJ

【 摘 要 】

Fescue toxicosis impacts beef cattle production via reductions in weight gain and muscle development. Isoflavone supplementation has displayed potential for mitigating these effects. The objective of the current study was to evaluate isoflavone supplementation with fescue seed consumption on rumen and serum metabolomes. Angus steers (n = 36) were allocated randomly in a 2 × 2 factorial arrangement of treatments including endophyte-infected (E+) or endophyte-free (E−) tall fescue seed, with (P+) or without (P−) isoflavones. Steers were provided a basal diet with fescue seed for 21 days, while isoflavones were orally administered daily. Following the trial, blood and rumen fluid were collected for metabolite analysis. Metabolites were extracted and then analyzed by UPLC-MS. The MAVEN program was implemented to identify metabolites for MetaboAnalyst 4.0 and SAS 9.4 statistical analysis. Seven differentially abundant metabolites were identified in serum by isoflavone treatment, and eleven metabolites in the rumen due to seed type (p < 0.05). Pathways affected by treatments were related to amino acid and nucleic acid metabolism in both rumen fluid and serum (p < 0.05). Therefore, metabolism was altered by fescue seed in the rumen; however, isoflavones altered metabolism systemically to potentially mitigate detrimental effects of seed and improve animal performance.

【 授权许可】

Unknown