Estimating the Product Inhibition Constant from Enzyme Kinetic Equations Using the Direct Linear Plot Method in One-Stage Treatment

Catalysts

卷:10

PedroL. Valencia¹  Diego Gajardo²  Bastián Sepúlveda²  Carolina Astudillo-Castro³ 

[1] Department of Chemical and Environmental Engineering, Universidad Técnica Federico Santa María, P.O. Box 110-V, Valparaíso 2390123, Chile;

[2] Department of Mathematics, Universidad Técnica Federico Santa María, Valparaíso 2390123, Chile;

[3] School of Food Engineering, Pontificia Universidad Católica de Valparaíso, Valparaíso 2360100, Chile;

关键词: direct linear plot;    median method;    product inhibition;    kinetic constants;    non-parametric;    distribution-free method;   

DOI  :  10.3390/catal10080853

来源: DOAJ

【 摘 要 】

A direct linear plot was applied to estimate kinetic constants using the product’s competitive inhibition equation. The challenge consisted of estimating three kinetic constants, V_max, K_m, and K_p, using two independent variables, substrates, and product concentrations, in just one stage of mathematical treatment. The method consisted of combining three initial reaction rate data and avoiding the use of the same three product concentrations (otherwise, this would result in a mathematical indetermination). The direct linear plot method was highly superior to the least-squares method in terms of accuracy and robustness, even under the addition of error. The direct linear plot method is a reliable and robust method that can be applied to estimate K_p in inhibition studies in pharmaceutical and biotechnological areas.

【 授权许可】

Unknown