Stress-Inducible Overexpression of SlDDF2 Gene Improves Tolerance against Multiple Abiotic Stresses in Tomato Plant

Horticulturae

卷:8

Rabea Al-Sayaydeh¹  Taghleb Al-Deeb²  Mohammad Abo Gamar³  Hmoud Al-Debei⁴  Ayed M. Al-Abdallat⁴  Najib El-Assi⁴ 

[1] Department of Agriculture Sciences, Shoubak College, Al-Balqa Applied University, Al-Salt 19117, Jordan;

[2] Department of Biological Sciences, Faculty of Science, Al al-Bayt University, Mafraq 25113, Jordan;

[3] Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid 21163, Jordan;

[4] Department of Horticulture and Crop Science, School of Agriculture, The University of Jordan, Amman 11942, Jordan;

关键词: abiotic stresses;    bioinformatics;    DREB1;    stress-inducible promoter;    tomato;    transcription factor;   

DOI  :  10.3390/horticulturae8030230

来源: DOAJ

【 摘 要 】

Dehydration-responsive element-binding protein 1 (DREB1)/C-repeat binding factor (CBF) family plays a key role in plant tolerance against different abiotic stresses. In this study, an orthologous gene of the DWARF AND DELAYED FLOWERING (DDF) members in Arabidopsis, SlDDF2, was identified in tomato plants. The SlDDF2 gene expression was analyzed, and a clear induction in response to ABA treatment, cold, salinity, and drought stresses was observed. Furthermore, two transgenic lines (SlDDF2-IOE#6 and SlDDF2-IOE#9) with stress-inducible overexpression of SlDDF2 under Rd29a promoter were generated. Under stress conditions, the gene expression of SlDDF2 was significantly higher in both transgenic lines. The growth performance, as well as physiological parameters, were evaluated in wild-type and transgenic plants. The transgenic lines showed growth retardation phenotypes and had higher chlorophyll content under stress conditions in plants. However, the relative decrease in growth performance (plant height, leaf number, and leaf area) in stressed transgenic lines was lower than that in stressed wild-type plants, compared with nonstressed conditions. The reduction in the relative water content and water loss rate was also lower in the transgenic lines. Compared with wild-type plants, transgenic lines showed enhanced tolerance to different abiotic stresses including water deficit, salinity, and cold. In conclusion, stress-inducible expression of SlDDF2 can be a useful tool to improve tolerance against multiple abiotic stresses in tomato plants.

【 授权许可】

Unknown