BMP-2 combined with salvianolic acid B promotes cardiomyocyte differentiation of rat bone marrow mesenchymal stem cells

Kaohsiung Journal of Medical Sciences

卷:33

Yang Lv¹  Hai-Ping Wang¹  Hao-Yu Wang¹  Bo Liu²  Chen-Wei Gao³ 

[1]Department of Histology and Embryology, Hebei North University, Zhangjiakou, China

[2]|Department of Pathology, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China

[3]|Department of Ultrasound, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China

关键词: Bone marrow mesenchymal stem cell;    Bone morphogenetic protein 2;    Salvianolic acid B;    Cardiomyocytes;    Differentiation;   

DOI  :  10.1016/j.kjms.2017.06.006

来源: DOAJ

【 摘 要 】

The present study tested the hypotheses that bone morphogenetic protein 2(BMP-2) combined with salvianolic acid B(Sal-B) enhance the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) towards cardiomyocytes in vitro. BMSCs were treated with BMP-2 and Sal-B, alone or in combination, for 72 h, then added new media (excluding inductive substance) and cultured for 4 weeks. The morphologic characteristics, surface antigens and mRNA expression of several transcription factors were also assessed. We found that they could all be identified by the positive staining for cardiomyocyte-specific proteins, desmin and cardiac troponin T, in these cells. Furthermore, the mRNA expression of GATA-4 and Nkx2.5 genes was slightly increased on day 7, enhanced on day 14 and decreased on day 28 while α-MHC gene was not expressed on day 7, but expressed slightly on day 14 and enhanced on day 28. The expression of these cardiac-specific markers in treatment groups were all significantly higher than those in the control group, respectively (P < 0.05). Transmission electron microscopy showed that BMSCs in treatment groups all had myofilaments, z line-like substances and mitochondria. Taken together, these results indicate that BMP-2 combined with Sal-B promotes myocardial differentiation of BMSCs, which may represent a potential therapeutic strategy for the treatment of ischemic heart disease.

【 授权许可】

Unknown