Resistance Breeding of Common Bean Shapes the Physiology of the Rhizosphere Microbiome

Frontiers in Microbiology

卷:10

Miriam Gonçalves de Chaves¹  Mariley de Cassia Fonseca¹  Siu Mui Tsai¹  Lucas William Mendes²  Rodrigo Mendes³  Jos M. Raaijmakers⁴ 

[1] Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture CENA, University of São Paulo, Piracicaba, Brazil;

[2] Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, Netherlands;

[3] Embrapa Meio Ambiente, Jaguariúna, Brazil;

[4] Institute of Biology Leiden, Leiden University, Leiden, Netherlands;

关键词: plant-microbe interactions;    metatranscriptome;    Biolog EcoPlate;    carbohydrate metabolism;    nutrient metabolism;    resistome;   

DOI  :  10.3389/fmicb.2019.02252

来源: DOAJ

【 摘 要 】

The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of Fusarium-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the Fusarium-resistant accession was distinctly different from that of the Fusarium-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community toward a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections.

【 授权许可】

Unknown