Furfural Produces Dose-Dependent Attenuating Effects on Ethanol-Induced Toxicity in the Liver

Frontiers in Pharmacology

卷:13

Zixin Zhu¹  Xuanmei Luo²  Zhuo Cheng³  Yonghui Huang³  Xiue Yan³ 

[1] Department of Infectious Diseases, The Affiliated Hospital of Guizhou Medical University, Guiyang, China;

[2] National Center of Gerontology, Beijing Hospital, Beijing, China;

[3] The Department of Gastroenterology and Hepatology, Peking University Third Hospital, Beijing, China;

关键词: furfural;    NAD+;    mitochondrial function;    PI3K-Akt pathway;    alcohol-associated liver disease;   

DOI  :  10.3389/fphar.2022.906933

来源: DOAJ

【 摘 要 】

Background: Alcohol-associated liver disease (ALD) increases the health burden worldwide, but effective drugs to prevent ALD are lacking. Furfural is a small molecule that can limit alcohol production in microorganisms and may have the capacity to attenuate ethanol-induced toxicity.Methods: Human HepG2 cells were incubated with ethanol and furfural, and cell viability, NAD+/NADH ratio, and mitochondrial function assays were performed. RNA sequencing (RNA-seq) data were used to annotate enriched pathways, and these findings were confirmed by reverse transcription-quantitative PCR (RT–qPCR) and Western blotting. C57BL/6J mice were fed a Lieber-DeCarli liquid diet. After 4 weeks, biochemical analysis of mouse serum and histological analysis of mouse livers were performed.Results: Different concentrations of furfural exerted different effects on mitochondria: low-dose furfural reduced reactive oxygen species (ROS) production, maintained mitochondrial transmembrane potential, and inhibited apoptosis pathway activation, while high-dose furfural led to the opposite effects. In mice, furfural mitigated transaminase increases and attenuated the lipid metabolism disorder that had been induced by ethanol.Conclusion: Low-dose furfural reduced ethanol-induced toxicity in the liver. Consuming food or beverages containing the appropriate level of furfural when drinking alcohol may be a convenient and useful way to prevent ALD.

【 授权许可】

Unknown