Prevention of Histological Changes after Colonic Diversion in Rats: An Experimental Study

Journal of Neonatal Surgery

卷:6

M Srinivas¹  A Shariff¹  M Pathak² 

[1] ;

[2] All India Institute of Medical sciences, Jodhpur;

关键词: Histology;    Colonic diversion;    Glutamine;    Psyllium;    Short Chain Fatty Acids;    Maharishi Amrit Kalash;   

DOI  :  10.21699/jns.v6i2.511

来源: DOAJ

【 摘 要 】

AIM: To determine the beneficial effects of Glutamine, Psyllium, Short Chain Fatty Acids (SCFA), and Maharishi Amrit Kalash (MAK), in preventing the histological changes after diversion colostomy.MATERIAL AND METHODS: After ethical clearance, male wistar rats (n=40) underwent diversion colostomy. Rats were divided into five groups of 8 rats each. Each group was given, after diversion colostomy, per rectally, one of the five agents being tested as an enema (3 ml/kg/day). Group I: Normal saline. Group II: Glutamine Group III: Psyllium, Group IV: MAK. Group V: Short chain fatty acid. The rats were euthanised 45 days after performing diversion colostomy. Morphometrical analysis of defunctionalised colon was done. Statistical analysis was done using SSPS statistical analysis software.RESULTS: On comparison with Group I epithelial cell height and mucosal thickness was significantly higher in Group II. Muscularis externae thickness was significantly higher in Group III on comparison with Group I. Group V had least inflammatory changes. CONCLUSIONS: Atrophic and inflammatory changes in the diverted colon can be prevented by per rectal administration of Glutamine, Psyllium and Short chain fatty acids.

【 授权许可】

Unknown