RT-LAMP-Based Molecular Diagnostic Set-Up for Rapid Hepatitis C Virus Testing

Biosensors

卷:12

Massimo Caputi¹  Sandhya Sharma²  Waseem Asghar²  Emmanuel Thomas³ 

[1] Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL 33431, USA;

[2] Department of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, FL 33431, USA;

[3] Department of Microbiology and Immunology and Sylvester Comprehensive Cancer Center, University of Miami School of Medicine, Miami, FL 33136, USA;

关键词: RT-LAMP;    point-of-care diagnostics;    microfluidics;    molecular HCV test;    colorimetric detection;   

DOI  :  10.3390/bios12050298

来源: DOAJ

【 摘 要 】

Hepatitis C virus (HCV) infections occur in approximately 3% of the world population. The development of an enhanced and extensive-scale screening is required to accomplish the World Health Organization’s (WHO) goal of eliminating HCV as a public health problem by 2030. However, standard testing methods are time-consuming, expensive, and challenging to deploy in remote and underdeveloped areas. Therefore, a cost-effective, rapid, and accurate point-of-care (POC) diagnostic test is needed to properly manage the disease and reduce the economic burden caused by high case numbers. Herein, we present a fully automated reverse-transcription loop-mediated isothermal amplification (RT-LAMP)-based molecular diagnostic set-up for rapid HCV detection. The set-up consists of an automated disposable microfluidic chip, a small surface heater, and a reusable magnetic actuation platform. The microfluidic chip contains multiple chambers in which the plasma sample is processed. The system utilizes SYBR green dye to detect the amplification product with the naked eye. The efficiency of the microfluidic chip was tested with human plasma samples spiked with HCV virions, and the limit of detection observed was 500 virions/mL within 45 min. The entire virus detection process was executed inside a uniquely designed, inexpensive, disposable, and self-driven microfluidic chip with high sensitivity and specificity.

【 授权许可】

Unknown