Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel

Genes

卷:12

Ales Pecinka¹  PranavPankaj Sahu¹  Anna Nowicka¹  Martin Kovacik¹  Barbara Tokarz²  Dorota Weigt³  Tamar Krugman⁴ 

[1] Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Czech Academy of Sciences, Šlechtitelů 31, 779 00 Olomouc, Czech Republic;

[2] Department of Botany, Physiology and Plant Protection, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland;

[3] Department of Genetics and Plant Breeding, Poznan University of Life Sciences, 11 Dojazd St., 60-632 Poznan, Poland;

[4] Institute of Evolution, University of Haifa, Abba Khoushy Ave. 199, Haifa 3498838, Israel;

关键词: Endoreduplication;    endosperm;    Hordeum vulgare ubsp. spontaneum;    seed development;    super cycle value;   

DOI  :  10.3390/genes12050711

来源: DOAJ

【 摘 要 】

Wild barley is abundant, occupying large diversity of sites, ranging from the northern mesic Mediterranean meadows to the southern xeric deserts in Israel. This is also reflected in its wide phenotypic heterogeneity. We investigated the dynamics of DNA content changes in seed tissues in ten wild barley accessions that originated from an environmental gradient in Israel. The flow cytometric measurements were done from the time shortly after pollination up to the dry seeds. We show variation in mitotic cell cycle and endoreduplication dynamics in both diploid seed tissues (represented by seed maternal tissues and embryo) and in the triploid endosperm. We found that wild barley accessions collected at harsher xeric environmental conditions produce higher proportion of endoreduplicated nuclei in endosperm tissues. Also, a comparison of wild and cultivated barley strains revealed a higher endopolyploidy level in the endosperm of wild barley, that is accompanied by temporal changes in the timing of the major developmental phases. In summary, we present a new direction of research focusing on connecting spatiotemporal patterns of endoreduplication in barley seeds and possibly buffering for stress conditions.

【 授权许可】

Unknown