Dual role of immunomodulation by crude polysaccharide from okra against carcinogenic liver injury in mice

Heliyon

卷:7

Sri Puji Astuti Wahyuningsih¹  Dwi Winarni¹  Ruey-an Doong²  Win Darmanto²  Raden Joko Kuncoroningrat Susilo²  Suhailah Hayaza³  Saikhu Akhmad Husen³ 

[1] Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Kampus C Universitas Airlangga, Surabaya 60115, Indonesia;

[2] Laboratory of Animal Physiology, Faculty of Science and Technology, Universitas Airlangga, Kampus C Universitas Airlangga, Surabaya 60115, Indonesia;

关键词: Okra;    Raw polysaccharide extract;    Diethylnitrosamine;    Immunomodulator;    Liver injury;   

DOI  :  

来源: DOAJ

【 摘 要 】

Polysaccharides have long been recognized as the anticancer agent with low toxicity and slight side effects. Okra (Abelmoschus esculentus L.), a flowering plant from the Malvaceae family that is found in tropical, subtropical, and warm temperate regions around the world. Hence, no in vivo studies have addressed the anticancer and immunomodulatory potential of polysaccharides from okra pods grown in Indonesia. This study aims to investigate the effect of okra raw polysaccharide extract (ORPE) to immune cells and cytokines of mice with hepatocarcinogenic conditions induced by diethylnitrosamine (DEN). Thirty-six male mice (BALB/C, 3–4 months old) were divided into six groups: the normal control group (CN), negative control (C-), positive control giving doxorubicin (C+), and three groups of ORPE treatment given the dose of 50 (P1), 100 (P2) and 200 (P3) mg/kg body weight. The administration of ORPE directly suppressed the regulatory T cells accumulations, suppressed macrophages activations, and balanced the number of effector T cells. However, it promoted CD8+ T cell activation at a low dose and increased interleukin-2 levels at all doses. These results suggest that ORPE has unique dual-functions as immunosuppressant and immunostimulant which can be a foundation for the application of the ORPE in the nutraceutical and pharmaceutical industries.

【 授权许可】

Unknown