STAR Protocols | 卷:3 |
Protocol for evaluating CD8+ T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy | |
Yuxian He1 Zhe Cong2 Yuting Sun3 Qiang Wei3 Jiahan Lu3 Ling Tong3 Jing Xue3 Jingjing Zhang3 Ting Chen3 | |
[1] Center for AIDS Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China; | |
[2] NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China; | |
关键词: Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Microbiology; Model Organisms; Molecular Biology; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Summary: Strong cellular immunity contributes to the control of HIV infection. Here, we describe a step-by-step protocol to assess the simian immunodeficiency virus (SIV)-specific CD8+ T cell responses by quantifying the degranulation, cytokine and chemokine production from SHIVSF162P3-infected rhesus macaques with an HIV fusion-inhibitory lipopeptide (LP-98) monotherapy. We also present the steps for adoptive transfer of an anti-CD8 antibody into a stable virologic control (SVC) group of LP-98-treated monkeys, confirming a direct role of CD8+ T cells in SVC macaques.For complete details on the use and execution of this protocol, please refer to Xue et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
【 授权许可】
Unknown