Genome and transcriptome analysis of rock-dissolving Pseudomonas sp. NLX-4 strain

Bioresources and Bioprocessing

卷:9

Miaojing Meng¹  Jinchi Zhang¹  Feifei Chen¹  Yanwen Wu¹  Wensheng Qin²  Ayyappa Kumar Sista Kameshwar²  Bo Zhang³ 

[1] Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Province Key Laboratory of Soil and Water Conservation and Ecological Restoration, Nanjing Forestry University;

[2] Department of Biology, Lakehead University;

[3] Department of Biology, University of Miami;

关键词: Rock mining;    Ecological restoration;    Silicate rock-dissolution;    Pseudomonas sp. NLX-4 strain;    Genome sequencing;    Transcriptome sequencing;   

DOI  :  10.1186/s40643-022-00548-w

来源: DOAJ

【 摘 要 】

Abstract Microbial weathering processes can significantly promote soil properties and reduce rock-to-soil ratio. Some soil-inhabiting bacteria exhibit efficient rock-dissolution abilities by releasing organic acids and other chemical elements from the silicate rocks. However, our understanding of the molecular mechanisms involved during bacterial rock-dissolution is still limited. In this study, we performed silicate rock-dissolution experiments on a Pseudomonas sp. NLX-4 strain isolated from an over-exploited mining site. The results revealed that Pseudomonas sp. NLX-4 strain efficiently accelerates the dissolution of silicate rocks by secreting amino acids, exopolysaccharides, and organic acids. Through employing genome and transcriptome sequencing (RNA-seq), we identified the major regulatory genes. Specifically, 15 differentially expressed genes (DEGs) encoding for siderophore transport, EPS and amino acids synthesis, organic acids metabolism, and bacterial resistance to adverse environmental conditions were highly up-regulated in silicate rock cultures of NLX-4 strain. Our study reports a potential bacterial based approach for improving the ecological restoration of over-exploited rock mining sites. Graphical Abstract

【 授权许可】

Unknown