Fine Mapping of Lr49 Using 90K SNP Chip Array and Flow-Sorted Chromosome Sequencing in Wheat

Frontiers in Plant Science

卷:10

Pippa Kay¹  Naeela Qureshi¹  Kerrie Forrest¹  Harbans S. Bariana²  Vallence Nsabiyera²  Deepak Baranwal²  Urmil K. Bansal²  Jaroslav Doležel³  Miroslav Valárik³  Matthew J. Hayden⁴ 

[1] Agriculture Victoria Research, AgriBio, Bundoora, VIC, Australia;

[2] Faculty of Science, School of Life Sciences and Environment, The University of Sydney Plant Breeding Institute, Cobbitty, NSW, Australia;

[3] Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czechia;

[4] School of Applied Systems Biology, La Trobe University, Bundoora, VIC, Australia;

关键词: adult plant resistance;    chromosome sorting;    Infinium iSelect 90K SNP array;    leaf rust;    marker assisted breeding;   

DOI  :  10.3389/fpls.2019.01787

来源: DOAJ

【 摘 要 】

Leaf rust, caused by Puccinia triticina, threatens global wheat production due to the constant evolution of virulent pathotypes that defeat commercially deployed all stage-resistance (ASR) genes in modern cultivars. Hence, the deployment of combinations of adult plant resistance (APR) and ASR genes in new wheat cultivars is desirable. Adult plant resistance gene Lr49 was previously mapped on the long arm of chromosome 4B of cultivar VL404 and flanked by microsatellite markers barc163 (8.1 cM) and wmc349 (10.1 cM), neither of which was sufficiently closely linked for efficient marker assisted selection. This study used high-density SNP genotyping and flow sorted chromosome sequencing to fine-map the Lr49 locus as a starting point to develop a diagnostic marker for use in breeding and to clone this gene. Marker sunKASP_21 was mapped 0.4 cM proximal to Lr49, whereas a group of markers including sunKASP_24 were placed 0.6 cM distal to this gene. Testing of the linked markers on 75 Australian and 90 European cultivars with diverse genetic backgrounds showed that sunKASP_21 was most strongly associated with Lr49. Our results also show that the Lr49 genomic region contains structural variation relative to the reference stock Chinese Spring, possibly an inverted genomic duplication, which introduces a new set of challenges for the Lr49 cloning.

【 授权许可】

Unknown