A fast and efficient preparative method for separation and purification of main bioactive xanthones from the waste of Garcinia mangostana L. by high-speed countercurrent chromatography

Arabian Journal of Chemistry

卷:14

Jaisielle Kelem F. Benjamim¹  Alberdan Silva Santos²  Wandson B. de Souza Pinheiro²  Francilia Campos de Siqueira³  Ricardo Felipe Alexandre de Mello⁴  Renan Campos Chisté⁴ 

[1] Corresponding authors at: Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity, Institute of Exact and Natural Sciences, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil.;

[2] Institute of Exact and Natural Sciences, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil;

[3] Faculty of Chemical Engineering, Institute of Technology, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil;

[4] Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil;

关键词: Bioactivity;    Mangosteen;    Agricultural by-products;    Antioxidant capacity;    Counter current chromatography separation;   

DOI  :  

来源: DOAJ

【 摘 要 】

In this work, we present a preparative elution method by high-speed countercurrent chromatography (Prep-HSCCC) for the separation and isolation of the main bioactive xanthones present in the alcoholic extract of Garcinia mangostana L. agro-industrial waste. The method proved to be quite efficient, operating with a two-phase solvent system consisting of Methanol/water/Ethanol/Hexane/methyl tert-butyl ether (6:3:1:6:4v/v), tail-head elution mode, with mobile phase flow of 5 mL/min and rotation speed of 800 rpm. The method demonstrated repeatability (RSD = 3.8–6.5%) and allowed the isolation of α-mangostin and γ-mangostin xanthones, both with purities above 93%, in a single step, performed in a time of 35 min. Isolated substances were identified by HPLC-PDA-MS and 1H and 13C NMR spectroscopy. Both xanthones were efficient scavengers of DPPH·· and ABTS·+ radicals in a concentration-dependent manner with EC50 at 8.43 and 3.31 µg/mL, respectively, for γ-mangostin, and 71.10 and 29.33 µg/mL, respectively, for α-mangostin.

【 授权许可】

Unknown