Sensitivity Validation of EWOD Devices for Diagnosis of Early Mortality Syndrome (EMS) in Shrimp Using Colorimetric LAMP–XO Technique

Sensors

卷:21

Wansika Kiatpathomchai¹  Jantana Kampeera¹  Kreeta Sukthang²  Wishsanuruk Wechsatol²  Adisorn Tuantranont³  Chakrit Sriprachuabwong³  Eakkachai Pengwang⁴ 

[1] Bioengineering and Sensing Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand;

[2] Department of Mechanical Engineering, King Mongkut’s University of Technology Thonburi, 126 Prachautid Rd, Bandmod, Thungkru, Bangkok 10140, Thailand;

[3] Graphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand;

[4] Institute of Field Robotics, King Mongkut’s University of Technology Thonburi, 126 Prachautid Rd, Bandmod, Thungkru, Bangkok 10140, Thailand;

关键词: droplet manipulation;    detection sensitivity;    colorimetric LAMP–XO;    electrowetting-on-dielectric (EWOD);    lab-on-a-chip (LOC);   

DOI  :  10.3390/s21093126

来源: DOAJ

【 摘 要 】

Electrowetting-on-dielectric (EWOD) is a microfluidic technology used for manipulating liquid droplets at microliter to nanoliter scale. EWOD has the ability to facilitate the accurate manipulation of liquid droplets, i.e., transporting, dispensing, splitting, and mixing. In this work, EWOD fabrication with suitable and affordable materials is proposed for creating EWOD lab-on-a-chip platforms. The EWOD platforms are applied for the diagnosis of early mortality syndrome (EMS) in shrimp by utilizing the colorimetric loop-mediated isothermal amplification method with pH-sensitive xylenol orange (LAMP–XO) diagnosis technique. The qualitative sensitivity is observed by comparing the limit of detection (LOD) while performing the LAMP–XO diagnosis test on the proposed lab-on-a-chip EWOD platform, alongside standard LAMP laboratory tests. The comparison results confirm the reliability of EMS diagnosis on the EWOD platform with qualitative sensitivity for detecting the EMS DNA plasmid concentration at 10² copies in a similar manner to the common LAMP diagnosis tests.

【 授权许可】

Unknown