Identification and Antioxidant Activity of the Extracts of Eugenia uniflora Leaves. Characterization of theAnti-Inflammatory Properties of Aqueous Extract on Diabetes Expression in an Experimental Model of Spontaneous Type 1 Diabetes (NOD Mice)

Antioxidants

卷:4

Laura Maria Molina Meletti¹  Cinthia Baú Betim Cazarin²  Marcelo Alexandre Prado²  Talita Cristina Colomeu³  Ricardo de Lima Zollner³  Nayara Simon Gonzalez Schumacher³  Daniella de Figueiredo³  Virginia de Campos Carvalho³ 

[1] Agronomic Institute of Campinas, Theodureto de Almeida Camargo, 1500, Vila Nova,13012-970 Campinas-SP, Brazil;

[2] Department of Food and Nutrition, School of Food Engineering, University of Campinas(FEA-Unicamp), Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz,13083-862 Campinas-SP, Brazil;

[3] Laboratory of Translational Immunology, Faculty of Medical Sciences, University of Campinas,Rua Vital Brazil, 300, Cidade Universitária Zeferino Vaz, 13083-888 Campinas-SP, Brazil;

关键词: Eugenia uniflora;    type 1 diabetes mellitus;    NOD mice;    antioxidant;   

DOI  :  10.3390/antiox4040662

来源: DOAJ

【 摘 要 】

Medical and folklore reports suggest that Eugenia uniflora (E. uniflora) is a functional food that contains numerous compounds in its composition, with anti-inflammatory, antioxidant and anti-diabetic effects. In the present study, we investigated the best solvents (water, ethanol and methanol/acetone) for extracting bioactive compounds of E. uniflora leaves, assessing total phenols and the antioxidant activity of the extracts by2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP),2,2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and Oxygen Radical Absorbance Capacity (ORAC) assays, identifying hydrolysable tannins and three phenolic compounds (ellagic acid, gallic acid and rutin) present in the leaves. In addition, we evaluated the incidence of diabetes, degree of insulitis, serum insulin, hepatic glutathione and tolerance test glucose in non-obese diabetic (NOD) mice. Our results suggest that the aqueous extract presents antioxidant activity and high total phenols, which were used as a type 1 diabetes mellitus (DM-1) treatment in NOD mice. We verified that the chronic consumption of aqueous extract reduces the inflammatory infiltrate index in pancreatic islets, maintaining serum insulin levels and hepatic glutathione, and reducing serum lipid peroxidation as well as the risk for diabetes.

【 授权许可】

Unknown