Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference alleles

Acta Agriculturae Slovenica

卷:111

Bouchaib KHADARI¹  Sylvain SANTONI¹  Jernej JAKŠE²  Dunja BANDELJ³  Matjaž HLADNIK³ 

[1] National Institute of Agricultural Research (INRA), UMR Genetic Improvement and Adaptation of Mediterranean and Tropical Plants AGAP 1334, F-34070 Montpellier, France;

[2] University of Ljubljana, Biotechnical Faculty, Agronomy Department, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia;

[3] University of Primorska, Faculty of Mathematics, Natural Sciences and Information Technologies, Glagoljaška 8, SI-6000 Koper, Slovenia;

关键词: microsatellite marker;    reference genotype;    interlaboratory comparison;    Ficus carica L.;   

DOI  :  10.14720/aas.2018.111.1.14

来源: DOAJ

【 摘 要 】

Microsatellites have been identified as the marker of choice in plant genotyping projects. However, due to length discrepancies obtained between different laboratories for the same allele, interlaboratory comparison of fingerprinting results is often a difficult task. The objectives of this study were to compare genotyping results of two laboratories, to evaluate genetic parameters of microsatellite markers and to determine reference allele sizes for fig cultivars from the Istrian peninsula.

Genotyping results of ninety fig (Ficus carica L.) accessions were comparable between the laboratories despite differences observed when comparing electropherograms of different capillary electrophoresis systems. Differences in lengths of the same alleles were detected due to different PCR methods and laboratory equipment, but the distances between alleles of the same locus were preserved. However, locus FSYC01 exhibited one allele dropout which led to misidentification of 28 heterozygotes as homozygote individuals suggesting this locus as unreliable. Allele dropout was assigned to the tail PCR technology or to a touchdown PCR protocol.

Genotypes of twenty-four reference cultivars from the Istrian peninsula were confirmed by both laboratories. These results will contribute to the usage of markers with greater reliability, discrimination power and consequently, to more reliable standardization with other fig genotyping projects.

【 授权许可】

Unknown