Polysaccharide Fraction Extracted from Endophytic Fungus Trichoderma atroviride D16 Has an Influence on the Proteomics Profile of the Salvia miltiorrhiza Hairy Roots

Biomolecules

卷:9

Qian-liang Ming¹  Xin Zhai²  Wei Peng²  Ting Han²  Si-jia Wu²  Khalid Rahman³  Qing Zhang⁴  Lu-ping Qin⁵ 

[1] Department of Pharmacognosy, School of Pharmacy, Army Medical University, 30 Gaotanyan Street, Chongqing 400038, China;

[2] Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China;

[3] Faculty of Science, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UK;

[4] School of Pharmacy, Chengdu University of Traditional Chinese Medicine, No.1166 Liutai Avenue, Chengdu 611137, China;

[5] School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China;

关键词: polysaccharide fraction;    Trichoderma atroviride;    Salvia miltiorrhiza;    proteomics;    tanshinones;   

DOI  :  10.3390/biom9090415

来源: DOAJ

【 摘 要 】

Trichoderma atroviride develops a symbiont relationship with Salvia miltiorrhiza and this association involves a number of signaling pathways and proteomic responses between both partners. In our previous study, we have reported that polysaccharide fraction (PSF) of T. atroviride could promote tanshinones accumulation in S. miltiorrhiza hairy roots. Consequently, the present data elucidates the broad proteomics changes under treatment of PSF. Furthermore, we reported several previously undescribed and unexpected responses, containing gene expression patterns consistent with biochemical stresses and metabolic patterns inside the host. In summary, the PSF-induced tanshinones accumulation in S. miltiorrhiza hairy roots may be closely related to Ca²⁺ triggering, peroxide reaction, protein phosphorylation, and jasmonic acid (JA) signal transduction, leading to an increase in leucine-rich repeat (LRR) protein synthesis. This results in the changes in basic metabolic flux of sugars, amino acids, and protein synthesis, along with signal defense reactions. The results reported here increase our understanding of the interaction between T. atroviride and S. miltiorrhiza and specifically confirm the proteomic responses underlying the activities of PSF.

【 授权许可】

Unknown