Model experiments for establishment of in vitro culture by micrografting in apple

International Journal of Horticultural Science

卷:11

E. Jámbor-Benczúr¹  K. Magyar-Tábori²  J. Dobránszky²  I. Hudák² 

[1] Corvinus University, Department of Floriculture and Dendrology, H-1118 Budapest, Villányi út 35-43 ., Hungary;

[2] Research Centre of University of Debrecen, H-4401 Nyíregyháza, P.O.Box 12., Hungary;

关键词: culture establishment;    micrografts;    survival;    tissue browning;   

DOI  :  10.31421/IJHS/11/1/557

来源: DOAJ

【 摘 要 】

Micrografting was used in our experiments for establishment of in vitro culture from one rootstock (`JTE-F') and three scion cultivars (`Remo', 'Rewena' and `Reanda') of apple. Shoot tips of these cultivars were harvested from field and grafted onto in vitro rootstock cultivars. Their survival and development were studied. 42-93% of shoot tips survived and developed further depending on cultivar. Impermanent browning of sticking agar-agar could be observed in 21-25% of the micrografts depending on cultivars but discolouration of agar-agar ceased within one week and did not cause any death of shoot tips. We used micrografting successfully for establishment of in vitro culture from cultivars, from which earlier with conventional methods the culture establishment was not possible because of hard tissue browning. However, further studies are necessary to ensure the survival and development of shoots after removing them from micrografts.

【 授权许可】

Unknown