Genome-Wide Detection of Key Genes and Epigenetic Markers for Chicken Fatty Liver

International Journal of Molecular Sciences

卷:21

Maiqing Zheng¹  Yonghong Zhang¹  Xiaodong Tan¹  Qinghe Li¹  Ranran Liu¹  Jie Wen¹  Guiping Zhao¹  Siyuan Xing¹ 

[1] Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;

关键词: chicken;    fatty liver;    lipid metabolism;    epigenetics;    dna methylation;    long noncoding rna;   

DOI  :  10.3390/ijms21051800

来源: DOAJ

【 摘 要 】

Chickens are one of the most important sources of meat worldwide, and the occurrence of fatty liver syndrome (FLS) is closely related to production efficiency. However, the potential mechanism of FLS remains poorly understood. An integrated analysis of data from whole-genome bisulfite sequencing and long noncoding RNA (lncRNA) sequencing was conducted. A total of 1177 differentially expressed genes (DEGs) and 1442 differentially methylated genes (DMGs) were found. There were 72% of 83 lipid- and glucose-related genes upregulated; 81% of 150 immune-related genes were downregulated in fatty livers. Part of those genes was within differentially methylated regions (DMRs). Besides, sixty-seven lncRNAs were identified differentially expressed and divided into 13 clusters based on their expression pattern. Some lipid- and glucose-related lncRNAs (e.g., LNC_006756, LNC_012355, and LNC_005024) and immune-related lncRNAs (e.g., LNC_010111, LNC_010862, and LNC_001272) were found through a co-expression network and functional annotation. From the expression and epigenetic profiles, 23 target genes (e.g., HAO1, ABCD3, and BLMH) were found to be hub genes that were regulated by both methylation and lncRNAs. We have provided comprehensive epigenetic and transcriptomic profiles on FLS in chicken, and the identification of key genes and epigenetic markers will expand our understanding of the molecular mechanism of chicken FLS.

【 授权许可】

Unknown