Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization

Catalysts

卷:7

Dušan Chorvát¹  Vilém Neděla²  Eva Tihlaříková²  Alica Vikartovská³  Tomáš Krajčovič³  Peter Gemeiner³  Marek Bučko³  Martin Gericke⁴  Thomas Heinze⁴  Igor Lacík⁵  Lucia Uhelská⁵ 

[1] Department of Biophotonics, International Laser Centre, Ilkovičova 3, SK-841 04 Bratislava, Slovakia;

[2] Department of Electron Microscopy, Institute of Scientific Instruments of the ASCR v.v.i., Královopolská 147, CZ-612 64 Brno, Czech Republic;

[3] Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38 Bratislava, Slovakia;

[4] Institute of Organic Chemistry and Macromolecular Chemistry, Centre of Excellence for Polysaccharide Research, Friedrich Schiller University of Jena, Humboldtstrasse 10, D-07743 Jena, Germany;

[5] Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 41 Bratislava, Slovakia;

关键词: polyelectrolyte complex beads;    environmental scanning electron microscopy;    confocal laser scanning microscopy;    Baeyer-Villiger biooxidation;    cyclohexanone monoxygenase;    immobilization;    viable whole-cell biocatalyst;   

DOI  :  10.3390/catal7110353

来源: DOAJ

【 摘 要 】

A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. The morphology of polyelectrolyte complex beads was characterised by environmental scanning electron microscopy; the spatial distribution of polymers in the beads and cell viability were examined using confocal laser scanning microscopy, and the texture was characterised by the mechanical resistance measurements.

【 授权许可】

Unknown