Tacrolimus-Induced Neurotrophic Differentiation of Adipose-Derived Stem Cells as Novel Therapeutic Method for Peripheral Nerve Injury

Frontiers in Cellular Neuroscience

卷:15

Xiaojing Li³  Xiangyun Yao⁴  Yanhao Li⁴  Yuanming Ouyang⁴  Zhiwen Yan⁴  Cunyi Fan⁴ 

[1] Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China;

[2] Shanghai Engineering Research Center for Orthopaedic Material Innovation and Tissue Regeneration, Shanghai, China;

[3] TianXinFu (Beijing) Medical Appliance Co., Ltd., Beijing, China;

[4] Youth Science and Technology Innovation Studio, Shanghai Jiao Tong University School of Medicine, Shanghai, China;

关键词: tacrolimus;    adipose-derived stem cells;    neurotrophic differentiation;    nerve crush injury;    peripheral nerve regeneration;   

DOI  :  10.3389/fncel.2021.799151

来源: DOAJ

【 摘 要 】

Peripheral nerve injuries (PNIs) are frequent traumatic injuries across the globe. Severe PNIs result in irreversible loss of axons and myelin sheaths and disability of motor and sensory function. Schwann cells can secrete neurotrophic factors and myelinate the injured axons to repair PNIs. However, Schwann cells are hard to harvest and expand in vitro, which limit their clinical use. Adipose-derived stem cells (ADSCs) are easily accessible and have the potential to acquire neurotrophic phenotype under the induction of an established protocol. It has been noticed that Tacrolimus/FK506 promotes peripheral nerve regeneration, despite the mechanism of its pro-neurogenic capacity remains undefined. Herein, we investigated the neurotrophic capacity of ADSCs under the stimulation of tacrolimus. ADSCs were cultured in the induction medium for 18 days to differentiate along the glial lineage and were subjected to FK506 stimulation for the last 3 days. We discovered that FK506 greatly enhanced the neurotrophic phenotype of ADSCs which potentiated the nerve regeneration in a crush injury model. This work explored the novel application of FK506 synergized with ADSCs and thus shed promising light on the treatment of severe PNIs.

【 授权许可】

Unknown