Impact of the Cooking Process on Metabolite Profiling of Acanthocereus tetragonus, a Plant Traditionally Consumed in Mexico

Molecules

卷:27

Yenny Adriana Gómez-Aguirre¹  Emmanuel Cabañas-García²  Francisco Cruz-Sosa³  Jaqueline Cornejo-Campos⁴  Carlos Areche⁵  José Rodolfo Velázquez-Martínez⁶  Carolina Estefanía Chávez-Murillo⁷  Oscar Javier Ramos-Herrera⁷ 

[1] CONACyT Research Fellow-Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, Aguascalientes 20100, Mexico;

[2] Centro de Estudios Científicos y Tecnológicos No. 18, Instituto Politécnico Nacional, Calle Circuito del Gato No. 202, Col. Ciudad Administrativa, Zacatecas 98160, Mexico;

[3] Departamento de Biotecnología, Universidad Autónoma Metropolitana-Campus Iztapalapa, San Rafael Atlixco 186, Vicentina, Ciudad de México 09340, Mexico;

[4] Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, Aguascalientes 20100, Mexico;

[5] Departamento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago 7800024, Chile;

[6] División Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Teapa, km 25, Villahermosa 86280, Mexico;

[7] Unidad Profesional lnterdisciplinaria de Ingeniería, Campus Zacatecas, lnstituto Politécnico Nacional (UPllZ-lPN), Calle Circuito del Gato No. 202, Col. Ciudad Administrativa, Zacatecas 98160, Mexico;

关键词: marginalized communities;    secondary metabolites;    cacti;    edible cactus;    chromatography;    alternative food;   

DOI  :  10.3390/molecules27123707

来源: DOAJ

【 摘 要 】

Acanthocereus tetragonus (L.) Hummelinck is used as an alternative food source in some Mexican communities. It has been shown that the young stems of A. tetragonus provide crude protein, fiber, and essential minerals for humans. In this work, we analyzed the phytochemical profile, the total phenolic content (TPC), and the antioxidant activity of cooked and crude samples of A. tetragonus to assess its functional metabolite contribution to humans. The phytochemical profile was analyzed using Ultra-High-Performance Liquid Chromatography coupled to High-Resolution Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). Under the proposed conditions, 35 metabolites were separated and tentatively identified. Of the separated metabolites, 16 occurred exclusively in cooked samples, 6 in crude samples, and 9 in both crude and cooked samples. Among the detected compounds, carboxylic acids, such as threonic, citric, and malic acids, phenolic acids, and glycosylated flavonoids (luteolin-O-rutinoside) were detected. The TPC and antioxidant activity were analyzed using the Folin–Ciocalteu method and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical inhibition method, respectively. The TPC and antioxidant activity were significantly reduced in the cooked samples. We found that some metabolites remained intact after the cooking process, suggesting that A. tetragonus represents a source of functional metabolites for people who consume this plant species.

【 授权许可】

Unknown