Sodium caseinate increases the number of B lymphocytes in mouse

Biomédica: revista del Instituto Nacional de Salud

卷:37

Vanihamín Domínguez¹  Edelmiro Santiago-Osorio¹  Beatriz Torres¹  Itzen Aguiñiga²  Leticia Moreno³ 

[1] Centro de Estudios y Servicios en Salud, Universidad Veracruzana, Veracruz, México;

[2] Unidad Multidisciplinaria de Investigación, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, Ciudad de México, México;

[3] Unidad de Investigación en Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, México;

关键词: linfocitos B;    proliferación de la célula;    inflamación;    citosina;    citometría de flujo;    ensayo de inmunoadsorción enzimática;   

DOI  :  10.7705/biomedica.v37i4.3604

来源: DOAJ

【 摘 要 】

Introduction: Sodium caseinate, a casein salt, is a proinflammatory agent in mice, and it is able to induce granulopoiesis in vivo and to increase the production of cytokines, which is key for this biological process.Objective: To assess whether sodium caseinate is able to induce a biological effect on cells from lymphoid origin and the production of cytokines involved in this lineage in vivo.Materials and methods: We used female BALB /c mice from 8 to 12 weeks old. The animals were injected intraperitoneally (IP) with 1 ml of sodium caseinate (10% PBS w/v) four times every 48 hours. The B cell populations and the incorporation of BrdU were analyzed by flow cytometry. Detection of interleukin-7 was assessed by ELISA (Enzyme-Linked ImmunoSorbent Assay).Results: We established that after intraperitoneal injection, the number of B lymphocytes 220+ from the spleen of mice treated with sodium caseinate increased compared to those that only received the vehicle (89.01±1.03 vs 75.66 ± 2.08), and the same was observed with the incorporation of BrdU in B220 + cells (38.59±4.48 vs 11.82±1.04 respectively). We also established that the concentration of interleukin-7 (IL-7) in the serum of mice treated with sodium caseinate increased compared to those that only received the vehicle (62.1 ± 17.5 vs 26.9 ± 4.4 pg/ml).Conclusion: Sodium caseinate was able to increase the number of B lymphocytes in the spleen; it also induced IL-7 production, a cytokine that is key for the B cell lymphopoiesis.

【 授权许可】

Unknown