Use of Microwave Radiometry to Monitor Thermal Denaturation of Albumin

Frontiers in Physiology

卷:9

Vadim Y. Tatur²  Yuri Ivanov³  Rafael A. Galiullin³  Andrey F. Kozlov³  Tatyana O. Pleshakova³  Vadim S. Ziborov⁴  Sergey G. Vesnin⁵  Nina D. Ivanova⁶  Igor Goryanin⁹ 

[1] Biological Systems Unit, Okinawa Institute of Science and Technology, Okinawa, Japan;

[2] Foundation of Advanced Technologies and Innovations, Moscow, Russia;

[3] Institute of Biomedical Chemistry, Moscow, Russia;

[4] Joint Institute for High Temperatures of Russian Academy of Sciences (RAS), Moscow, Russia;

[5] Medical MicroWave Radiometry (MMWR) LTD, Edinburgh, United Kingdom;

[6] Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia;

[7] RES LTD, Moscow, Russia;

[8] School of Informatics, University of Edinburgh, Edinburgh, United Kingdom;

[9] Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China;

关键词: water clusters;    protein denaturation;    rate constant;    microwave emission;    temperature;   

DOI  :  10.3389/fphys.2018.00956

来源: DOAJ

【 摘 要 】

This study monitored thermal denaturation of albumin using microwave radiometry. Brightness Temperature, derived from Microwave Emission (BTME) of an aqueous solution of bovine serum albumin (0.1 mM) was monitored in the microwave frequency range 3.8–4.2 GHz during denaturation of this protein at a temperature of 56°C in a conical polypropylene cuvette. This method does not require fluorescent or radioactive labels. A microwave emission change of 1.5–2°C in the BTME of aqueous albumin solution was found during its denaturation, without a corresponding change in the water temperature. Radio thermometry makes it possible to monitor protein denaturation kinetics, and the resulting rate constant for albumin denaturation was 0.2 ± 0.1 min−1, which corresponds well to rate constants obtained by other methods.

【 授权许可】

Unknown