Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study

Materials

卷:15

Yoshiya Hashimoto¹  Ei Yamamoto²  Shigeki Hontsu²  Liji Chen³  Lin Ma⁴  Sifan Yan⁴  Xin Wang⁴  Satoshi Komasa⁴  Yuhao Zeng⁴  Min Li⁴  Joji Okazaki⁴  Yuanyuan Yang⁴  Mariko Nishizaki⁴ 

[1] Department of Biomaterials, Osaka Dental University, 8-1, Kuzuha Hanazono-cho, Hirakata 573-1121, Japan;

[2] Department of Biomedical Engineering, Faculty of Biology-Oriented Science and Technology, Kindai University, 930 Nishimitani, Kinokawa 649-6493, Japan;

[3] Department of Orthodontics, Osaka Dental University, 8-1, Kuzuha hanazono-cho, Hirakata-shi, Osaka 573-1121, Japan;

[4] Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuha hanazono-cho, Hirakata-shi, Osaka 573-1121, Japan;

关键词: Er:YAG laser;    pulsed laser deposition;    hydroxyapatite coating;    titanium implant;   

DOI  :  10.3390/ma15062306

来源: DOAJ

【 摘 要 】

The surface of titanium (Ti) dental implants must be modified to improve their applicability, owing to the biological inertness of Ti. This study aims to use sandblasting as a pretreatment method and prepare a hydroxyapatite (HA) coating on Ti to improve its biocompatibility and induce bone bonding and osteogenesis. In this paper, sandblasted Ti discs were coated with α-tricalcium phosphate (α-TCP) via Er:YAG pulsed laser deposition (Er:YAG-PLD). An HA coating was then obtained via the hydrothermal treatment of the discs at 90 °C for 10 h. The surface characteristics of the samples were evaluated by SEM, SPM, XPS, XRD, FTIR, and tensile tests. Rat bone marrow mesenchymal stem cells were seeded on the HA-coated discs to determine cellular responses in vitro. The surface characterization results indicated the successful transformation of the HA coating with a nanorod-like morphology, and its surface roughness increased. In vitro experiments revealed increased cell attachment on the HA-coated discs, as did the cell morphology of fluorescence staining and SEM analysis; in contrast, there was no increase in cell proliferation. This study confirms that Er:YAG-PLD could be used as an implant surface-modification technique to prepare HA coatings with a nanorod-like morphology on Ti discs.

【 授权许可】

Unknown