Inhalation of Atmospheric-Pressure Gas Plasma Attenuates Brain Infarction in Rats With Experimental Ischemic Stroke

Frontiers in Neuroscience

卷:16

Zhongfang Shi¹  Fang Yuan¹  Xu Yan¹  Lixin Xu¹  Ye Chen²  Kostya Ken Ostrikov³  Jiting Ouyang⁴  Bingyan Yang⁴  Ruoyu Han⁴ 

[1] Department of Pathophysiology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, Beijing, China;

[2] School of Basic Medical Sciences, Capital Medical University, Beijing, China;

[3] School of Chemistry and Physics and Centre for Biomedical Technologies, Queensland University of Technology, Brisbane, QLD, Australia;

[4] School of Physics, Beijing Institute of Technology, Beijing, China;

关键词: atmospheric pressure plasma;    plasma medicine;    ischemic stroke;    magnetic resonance imaging;    neuro-protection;   

DOI  :  10.3389/fnins.2022.875053

来源: DOAJ

【 摘 要 】

Previous studies suggest the potential efficacy of neuroprotective effects of gaseous atmospheric-pressure plasma (APP) treatment on neuronal cells. However, it remains unclear if the neuroprotective properties of the gas plasmas benefit the ischemic stroke treatment, and how to use the plasmas in the in vivo ischemic stroke models. Rats were subjected to 90 min middle cerebral artery occlusion (MCAO) to establish the ischemic stroke model and then intermittently inhaled the plasma for 2 min at 60 min MCAO. The regional cerebral blood flow (CBF) was monitored. Animal behavior scoring, magnetic resonance imaging (MRI), 2,3,5-triphenyltetrazolium chloride (TTC) staining, and hematoxylin and eosin (HE) staining were performed to evaluate the therapeutic efficacy of the gas plasma inhalation on MCAO rats. Intermittent gas plasma inhalation by rats with experimental ischemic stroke could improve neurological function, increase regional CBF, and decrease brain infarction. Further MRI tests showed that the gas plasma inhalation could limit the ischemic lesion progression, which was beneficial to improve the outcomes of the MCAO rats. Post-stroke treatment with intermittent gas plasma inhalation could reduce the ischemic lesion progression and decrease cerebral infarction volume, which might provide a new promising strategy for ischemic stroke treatment.

【 授权许可】

Unknown