The microbiological characteristics of Staphylococcus aureus isolated from patients with native valve infective endocarditis

Virulence

卷:10

Kyung-Hwa Park¹  Hee-Chang Jang²  Shinhye Cheon³  Shinwon Lee⁴  Chung-Jong Kim⁵  Sung-Min Kiem⁶  Yee Gyung Kwak⁷  Sun Hee Lee⁸  Eu Suk Kim⁹  Hong Bin Kim⁹  Kyoung-Ho Song⁹  Kyoung Un Park⁹  Pyoeng Gyun Choe¹⁰  Wan Beom Park¹⁰  Myoung-don Oh¹⁰  Nam Joong Kim¹⁰  Young Keun Kim¹¹ 

[1] Chonnam National University Hospital;

[2] Chonnam National University Hwasun Hospital;

[3] Chungnam National University School of Medicine;

[4] Daegu Fatima Hospital;

[5] Ewha Womans University Seoul Hospital;

[6] Inje University Haeundae Paik Hospital;

[7] Inje University Ilsan Paik Hospital;

[8] Pusan National University School of Medicine and Medical Research Institute, Pusan National University Hospital;

[9] Seoul National University Bundang Hospital;

[10] Seoul National University College of Medicine;

[11] Yonsei University Wonju College of Medicine;

关键词: staphylococcus aureus;    bacteremia;    infective endocarditis;    fibronectin;    single-nucleotide polymorphism;    virulence;   

DOI  :  10.1080/21505594.2019.1685631

来源: DOAJ

【 摘 要 】

The microbiological characteristics of Staphylococcus aureus causing infective endocarditis (IE) have not been investigated thoroughly. We compared the characteristics of S. aureus isolates from patients with and without IE. Cases of S. aureus bacteremia (SAB) were collected from 10 hospitals over 7 years. Cases of native valve IE were matched with non-IE controls according to the following criteria: central-line-associated infection, community-acquired infection, methicillin susceptibility, and if possible, the primary site of infection. Genes coding virulence factors were analyzed using multiplex polymerase chain reactions. Fibrinogen and fibronectin-binding properties were assessed using in vitro binding assays. The fibronectin-binding protein A gene (fnbpA) was sequenced. Of 2,365 cases of SAB, 92 had IE. After matching, 37 pairs of S. aureus isolates from the IE cases and non-IE controls were compared; fnbpA was detected in 91.9% of the IE isolates and 100% of the non-IE isolates (p = 0.24). While the fibrinogen binding ratio was similar (1.07 ± 0.33 vs. 1.08 ± 0.26, p = 0.89), the fibronectin-binding ratio was significantly higher in the IE-group (1.31 ± 0.42 vs. 1.06 ± 0.31, p = 0.01). The proportions of major single-nucleotide polymorphisms in fnbpA were as follows: E652D (2.9% vs. 2.7%), H782Q (65.6% vs. 60.6%), and K786N (65.6% vs. 72.7%). The fibronectin-binding ratio was positively correlated with the number of SNPs present in IE cases (p < 0.001) but not in the non-IE controls (p = 0.124). Fibronectin-binding might play a key role in SAB IE. However, the degree of binding may be mediated by genetic variability between isolates.

【 授权许可】

Unknown