Selective oncolytic effect in Epstein-Barr virus (EBV)-associated gastric carcinoma through efficient lytic induction by Euphorbia extracts

Journal of Functional Foods

卷:42

Sunhee Jung¹  Mingu Kang¹  Hyejeong Jeong¹  Sang-Bae Han¹  Jae-Hyoung Song¹  Janghwan Kim²  Hyunju Kang²  Sei-Ryang Oh²  Hyung Won Ryu²  Sungchan Cho³  Miri Choi³  Jiyeon Baek³  Hyojeung Kang³  Dong-eun Kim³  Heung Joo Yuk⁴ 

[1] Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do 28116, South Korea;

[2] College of Pharmacy, Chungbuk National University, 1 Chungdae-ro Seowon-gu, Cheongju-si, Chungcheongbuk-do 28644, South Korea;

[3] Anticancer Agent Research Center, Korea Research Institute of Bioscience &

[4] Natural Medicine Research Center, Korea Research Institute of Bioscience &

关键词: Epstein-Barr virus;    EBV-associated gastric carcinoma;    Lytic induction;    Medicinal plant extracts;    Euphorbia;   

DOI  :  

来源: DOAJ

【 摘 要 】

Lytic induction of latent Epstein-Barr virus (EBV) has been considered as a therapeutic approach for efficient treatment of EBV-associated malignancies. Here, we established a robust assay system quantitatively measuring EBV lytic induction and identified three Euphorbia extracts as strong inducers from a screen of Korean medicinal plant extracts library. Expression of lytic genes were efficiently induced by each Euphorbia extract in SNU-719 (EBV-positive gastric carcinoma cells). Euphorbia pekinensis, out of three extracts, exhibited the most potent lytic-inducing activity. Importantly, treatment with E. pekinensis extract led to an obvious cytotoxic effect on SNU-719 cells, but little on MKN-74 cells (EBV-negative gastric carcinoma cells), indicating the selective oncolytic effect on EBV-positive gastric carcinoma. We further revealed that lytic-inducing activity of E. pekinensis was mediated by PKC/MEK signal. Moreover, we defined two structural analogs of PMA with strong lytic-inducing activity, suggesting their therapeutic applicability for the treatment of EBV-associated gastric carcinoma.

【 授权许可】

Unknown