The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties

International Journal of Molecular Sciences

卷:18

Stephan Ensminger¹  Dirk Vordermark¹  Matthias Bache¹  Faramarz Dehghani²  Tim Hohmann²  Carolin Vogel²  Urszula Grabiec²  Chalid Ghadban² 

[1] Department of Radiation Oncology, Martin Luther University Halle-Wittenberg, Ernst-Grube-Strasse 40, 06120 Halle, Germany;

[2] Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse 52, 06108 Halle, Germany;

关键词: glioblastoma;    cell mechanics;    slice cultures;    actin;    phalloidin;    radiation;    cytoskeleton;   

DOI  :  10.3390/ijms18092001

来源: DOAJ

【 摘 要 】

Irradiation is the standard therapy for glioblastoma multiforme. Glioblastoma are highly resistant to radiotherapy and the underlying mechanisms remain unclear. To better understand the biological effects of irradiation on glioblastoma cells, we tested whether nonlethal irradiation influences the invasiveness, cell stiffness, and actin cytoskeleton properties. Two different glioblastoma cell lines were irradiated with 2 Gy and changes in mechanical and migratory properties and alterations in the actin structure were measured. The invasiveness of cell lines was determined using a co-culture model with organotypic hippocampal slice cultures. Irradiation led to changes in motility and a less invasive phenotype in both investigated cell lines that were associated with an increase in a ”generalized stiffness” and changes in the actin structure. In this study we demonstrate that irradiation can induce changes in the actin cytoskeleton and motility, which probably results in reduced invasiveness of glioblastoma cell lines. Furthermore, “generalized stiffness” was shown to be a profound marker of the invasiveness of a tumor cell population in our model.

【 授权许可】

Unknown