Dynamical Expression of MicroRNA-127-3p in Proliferating and Differentiating C2C12 Cells

Asian-Australasian Journal of Animal Sciences

卷:29

Peng Zhou¹  Gaofu Wang¹  Yongfu Huang¹  Hangxing Ren¹  Lin Wang¹  Liangjia Liu¹  Jing Jiang¹  Jinhong Zhao¹  Jie Li²  Youji Ma² 

[1] Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China;

[2] College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China;

关键词: MiR-127-3p;    C2C12;    Proliferation;    Differentiation;    Goats;   

DOI  :  10.5713/ajas.15.0968

来源: DOAJ

【 摘 要 】

MicroRNAs (miRNAs) are highly conserved, short non-coding RNAs that regulate gene expression at the posttranscriptional level. Although many miRNAs are identified in muscles and muscle cells, their individual roles are still not fully understood. In the present study, we investigated a muscle highly-expressed miRNA, miR-127-3p, in C2C12 myoblasts and tissues of goats with different muscle phenotypes (Boer vs Wushan black goats). Our results demonstrated that i) miR-127-3p was extensively expressed in tissues of goats; ii) miR-127-3p was higher expressed in muscle, spleen, heart, and skin in the muscular goats (Boer goats) than the control (Wushan black goats). Then we further characterized the dynamical expression of miR-127-3p, MyoD, MyoG, Myf5, Mef2c, and Myosin in the proliferating and differentiating C2C12 myoblasts at day of 0, 1, 3, 5, and 7 in culture mediums. Especially, we found that miR-127-3p was significantly higher expressed in the proliferating than differentiating cells. Our findings suggest that miR-127-3p probably plays roles in the proliferation and differentiation of myoblasts, which further underlies regulation of muscle phenotype in goats.

【 授权许可】

Unknown