Next Generation Sequencing for Long Non-coding RNAs Profile for CD4+ T Cells in the Mouse Model of Acute Asthma

Frontiers in Genetics

卷:10

Mingshun Zhang¹  Wenqin Yu²  Mao Huang³  Zhengxia Wang³  Chaojie Wu³  Ningfei Ji³  Zhongqi Chen³  Zhixiao Sun³  Fan Hu⁵ 

[1] Department of Immunology, Nanjing Medical University, Nanjing, China;

[2] Department of Infectious Disease, Taizhou People’s Hospital, Taizhou, China;

[3] Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China;

[4] NHC Key Laboratory of Antibody Technique, Nanjing Medical University, Nanjing, China;

[5] State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China;

关键词: asthma;    CD4+ T lymphocyte;    long non-coding RNA;    mRNA;    next-generation sequencing;   

DOI  :  10.3389/fgene.2019.00545

来源: DOAJ

【 摘 要 】

Background and AimsAlthough long non-coding RNAs (lncRNAs) have been linked to many diseases including asthma, little is known about lncRNA transcriptomes of CD4+ T cells in asthma. The present study aimed to explore the lncRNAs profile in the CD4+T cells from the mouse model of acute asthma.MethodsNext generation sequencing for lncRNAs and mRNAs was performed on CD4+ T cells from asthma and control mice. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway analyses were performed to predict the functions and signal pathways for the aberrant lncRNAs. The selected lncRNAs were further measured using quantitative real-time PCR (polymerase chain reaction) and observed in the fluorescence in situ hybridization (FISH). The lncRNA–mRNA co-expression network was constructed via Pearson’s correlation coefficient and Cytoscape 3.6.ResultsNext generation sequencing revealed 36 up-regulated lncRNAs and 98 down-regulated lncRNAs in acute asthma compared with controls. KEGG pathway analysis showed that cytokine-cytokine receptor interaction had the highest enrichment scores. A co-expression network was constructed in which 23 lncRNAs and 301 mRNAs altered formed a total of 12424 lncRNA and mRNA pairs. To validate the RNA sequencing results, we measured the 4 different lncRNAs using qPCR. The lncRNA fantom3_9230106C11 was significantly reduced in CD4+ T cells of asthma. Bioinformatics analysis showed that lncRNA fantom3_9230106C11 had the potential to interact with many miRNAs and transcription factors related to Th2 differentiation.ConclusionThis study provided the first evidence for different expression of lncRNAs of CD4+T cells in asthma and may serve as a template for further, larger functional in-depth analyses regarding asthma molecular lncRNAs.

【 授权许可】

Unknown