Silver Nanoparticles Using Eucalyptus or Willow Extracts (AgNPs) as Contact Lens Hydrogel Components to Reduce the Risk of Microbial Infection

Molecules

卷:26

Ioannis Sainis¹  Christina Papachristodoulou²  Panagiotis Zoumpoulakis³  Thomas Mavromoustakos⁴  Andreas K. Rossos⁵  Christina N. Banti⁵  Sotiris K. Hadjikakou⁵  Panagiotis K. Raptis⁵ 

[1] Cancer Biobank Center, University of Ioannina, 45110 Ioannina, Greece;

[2] Department of Physics, University of Ioannina, 45110 Ioannina, Greece;

[3] Laboratory of Chemistry, Analysis and Design of Food Processes, Department of Food Science and Technology, University of West Attica, 12243 Attica, Greece;

[4] Organic Chemistry Laboratory, Department of Chemistry, University of Athens Greece, 15571 Athens, Greece;

[5] Section of Inorganic and Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece;

关键词: silver nanoparticles;    eucalyptus leaves extract;    willow bark extract;    antimicrobial materials;    hydrogels;    contact lens;   

DOI  :  10.3390/molecules26165022

来源: DOAJ

【 摘 要 】

Eucalyptus leaves (ELE) and willow bark (WBE) extracts were utilized towards the formation of silver nanoparticles (AgNPs(ELE), AgNPs(WBE)). AgNPs(ELE) and AgNPs(WBE) were dispersed in polymer hydrogels to create pHEMA@AgNPs(ELE)_2 and pHEMA@AgNPs(WBE)_2 using hydroxyethyl-methacrylate (HEMA). The materials were characterized in a solid state by X-ray fluorescence (XRF) spectroscopy, X-ray powder diffraction analysis (XRPD), thermogravimetric differential thermal analysis (TG-DTA), differential scanning calorimetry (DTG/DSC) and attenuated total reflection spectroscopy (ATR-FTIR) and ultraviolet visible (UV-vis) spectroscopy in solution. The antimicrobial potential of the materials was investigated against the Gram-negative bacterial strain Pseudomonas aeruginosa (P. aeruginosa) and the Gram-positive bacterial strain of the genus Staphylococcus epidermidis (S. epidermidis) and Staphylococcus aureus (S. aureus), which are involved in microbial keratitis. The percentage of bacterial viability of P. aeruginosa and S. epidermidis upon their incubation over the pHEMA@AgNPs(ELE)_2 discs is interestingly low (28.3 and 6.8% respectively), while the inhibition zones (IZ) formed are 12.3 ± 1.7 and 13.2 ± 1.2 mm, respectively. No in vitro toxicity of this material towards human corneal epithelial cells (HCEC) was detected. Despite its low performance against S. aureus, pHEMA@AgNPs(ELE)_2 could be an efficient candidate towards the development of contact lenses that reduces microbial infection risk.

【 授权许可】

Unknown