Urine metabolite profiling of Indian Antarctic Expedition members: NMR spectroscopy-based metabolomic investigation

Heliyon

卷:7

Deepti Upadhyay¹  Uma Sharma¹  Naranamangalam Jagannathan¹  Brij Bhushan²  Lilly Ganju²  Shashi Bala Singh³ 

[1] All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India;

[2] Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Lucknow Road, Timarpur, New Delhi 110054, India;

[3] National Institute of Pharmaceutical Education and Research (NIPER), NH 9, Kukatpally Industrial Estate, Balanagar, Hyderabad, Telangana 500037, India;

关键词: Expedition;    Antarctic stress;    Metabolite;    NMR spectroscopy;    Metabolism;   

DOI  :  

来源: DOAJ

【 摘 要 】

The southernmost region of earth, Antarctica, has world's most challenging environments. Those who live for long time and work in Antarctic stations are subjected to environmental stresses such as cold weather, photoperiod variations leading to disrupted sleep cycles, constrained living spaces, dry air, non-availability of fresh food items, and high electromagnetic radiations, psychological factors, such as geographical and social isolation, etc. All these factors have a significant impact on the human body. The present study investigated the impact of Antarctica harsh environment on human physiology and its metabolic processes by evaluating urine metabolome, using 1H NMR spectroscopy and analyzing certain physiological and clinical parameters for correlation with physiological expression data and metabolite results. Two study groups - before Antarctic exposure (B) and after Antarctic exposure (E), consisting of 11 subjects, exposed to one-month summer expedition, were compared. 35 metabolites in urine samples were identified from the 700 MHz 1H NMR spectra from where integral intensity of 22 important metabolites was determined. Univariate analysis indicated significant decrease in the levels of citrate and creatinine in samples collected post-expedition. Multivariate analysis was also performed using 1H NMR spectroscopy, because independent metabolite abundances may complement each other in predicting the dependent variables. 10 metabolites were identified among the groups; the OPLS-DA and VIP score indicated variation in appearance of metabolites over different time periods with insignificant change in the intensities. Metabolite results illustrate the impact of environmental stress or altered life style including the diet with absence of fresh fruits and vegetables, on the pathophysiology of the human health. Metabolic adaptation to Antarctic environmental stressors may help to highlight the effect of short-term physiological status and provide important information during Antarctic expeditions to formulate management programmes.

【 授权许可】

Unknown