Magnetic tweezers: development and use in single-molecule research

BioTechniques

卷:72

Santosh Gaire¹  Abhijit Sarkar¹  Roberto Fabian¹  Ian Pegg¹ 

[1] 1Department of Physics, The Catholic University of America, 620 Michigan Avenue NE, Washington, DC 20064, USA;

关键词: Allan variance;    horizontal magnetic tweezers;    magnetic traps;    magnetic tweezers;    particle tracking;    power spectrum;   

DOI  :  10.2144/btn-2021-0104

来源: DOAJ

【 摘 要 】

The use of magnetic tweezers for single-molecule micromanipulation has evolved rapidly since its introduction approximately 30 years ago. Magnetic tweezers have provided important insights into the dynamic activity of DNA-processing enzymes, as well as detailed, high-resolution information on the mechanical properties of DNA. These successes have been enabled by major advancements in the hardware and software components of these devices. These developments now allow for a much richer mechanistic understanding of the functions and mechanisms of DNA-binding enzymes. In this review, the authors briefly discuss the fundamental principles of magnetic tweezers and describe the advancements that have made it a superlative tool for investigating, at the single-molecule level, DNA and its interactions with DNA-binding proteins.

【 授权许可】

Unknown