Comparison of Rapid Antigen Tests for COVID-19

Viruses

卷:12

Takayuki Ogura¹  Kensuke Fujita¹  Eisuke Adachi²  Hiroyuki Nagai²  Hiroaki Mitsuya³  Kenji Maeda³  Shin-ichiro Hattori³  Norio Omagari⁴  Kei Yamamoto⁴  Takato Nakamoto⁴  Michiko Koga⁵  Hiroshi Yotsuyanagi⁵  Makoto Saito⁵  Kazuhiko Ikeuchi⁵  Hidefumi Koh⁶  Takahiro Fukui⁶  Fumimaro Ito⁶  Yuri Furusawa⁷  Shinya Yamada⁷  Kiyoko Iwatsuki-Horimoto⁷  Seiya Yamayoshi⁷  Michiko Ujie⁷  Yoshihiro Kawaoka⁷  Masaki Imai⁷  Yuko Sakai-Tagawa⁷  Mutsumi Ito⁷  Atsuhiro Yasuhara⁷  Osamu Akasaka⁸  Rie Baba⁹  Ichiro Nakachi⁹ 

[1] Department of Emergency Medicine and Critical Care Medicine, Saiseikai Utsunomiya Hospital, Tochigi 321-0974, Japan;

[2] Department of Infectious Diseases and Applied Immunology, IMSUT Hospital of Institute of Medical Science, the University of Tokyo, Tokyo 108-8639, Japan;

[3] Department of Refractory Viral Infections, National Center for Global Health and Medicine Research Institute, Tokyo 162-8655, Japan;

[4] Disease Control and Prevention Center, National Center for Global Health and Medicine Hospital, Tokyo 162-8655, Japan;

[5] Division of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan;

[6] Division of Pulmonary Medicine, Department of Internal Medicine, Tachikawa Hospital, Tokyo 190-8531, Japan;

[7] Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan;

[8] Emergency Medical Center, Fujisawa City Hospital, Kanagawa 251-8550, Japan;

[9] Pulmonary Division, Department of Internal Medicine, Saiseikai Utsunomiya Hospital, Tochigi 321-0974, Japan;

关键词: rapid antigen test;    diagnosis;    COVID-19;    SARS-CoV-2;   

DOI  :  10.3390/v12121420

来源: DOAJ

【 摘 要 】

Reverse transcription-quantitative PCR (RT-qPCR)-based tests are widely used to diagnose coronavirus disease 2019 (COVID-19). As a result that these tests cannot be done in local clinics where RT-qPCR testing capability is lacking, rapid antigen tests (RATs) for COVID-19 based on lateral flow immunoassays are used for rapid diagnosis. However, their sensitivity compared with each other and with RT-qPCR and infectious virus isolation has not been examined. Here, we compared the sensitivity among four RATs by using severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolates and several types of COVID-19 patient specimens and compared their sensitivity with that of RT-qPCR and infectious virus isolation. Although the RATs read the samples containing large amounts of virus as positive, even the most sensitive RAT read the samples containing small amounts of virus as negative. Moreover, all RATs tested failed to detect viral antigens in several specimens from which the virus was isolated. The current RATs will likely miss some COVID-19 patients who are shedding infectious SARS-CoV-2.

【 授权许可】

Unknown