Data concerning the proteolytic resistance and oxidative stress in LAN5 cells after treatment with BSA hydrogels

Data in Brief

卷:9

Giovanna Navarra¹  Valeria Militello¹  Chiara Peres¹  Pier Luigi San Biagio²  Daniela Giacomazza²  Marco Contardi²  Marta Di Carlo³  Pasquale Picone³ 

[1] Dipartimento di Fisica e Chimica, Università di Palermo, Viale delle Scienze, Building 18, 90100 Palermo, Italy;

[2] Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Via U. La Malfa 153, 90146 Palermo, Italy;

[3] Istituto di Biomedicina e Immunologia Molecolare, Consiglio Nazionale delle Ricerche, Via U. La Malfa 153, 90146 Palermo, Italy;

关键词: Hydrogels;    β-aggregates;    Cell-scaffold;    Drug delivery;    Proteolytic resistance;    Oxidative Stress;   

DOI  :  10.1016/j.dib.2016.08.065

来源: DOAJ

【 摘 要 】

Proteolytic resistance is a relevant aspect to be tested in the formulation of new nanoscale biomaterials. The action of proteolytic enzymes is a very fast process occurring in the range of few minutes. Here, we report data concerning the proteolytic resistance of a heat-set BSA hydrogel obtained after 20-hour incubation at 60 °C prepared at the pH value of 3.9, pH at which the hydrogel presents the highest elastic character with respect to gel formed at pH 5.9 and 7.4 “Heat-and pH-induced BSA conformational changes, hydrogel formation and application as 3D cell scaffold” (G. Navarra, C. Peres, M. Contardi, P. Picone, P.L. San Biagio, M. Di Carlo, D. Giacomazza, V. Militello, 2016) [1]. We show that the BSA hydrogel produced by heating treatment is protected by the action of proteinase K enzyme. Moreover, we show that LAN5 cells cultured in presence of BSA hydrogels formed at pH 3.9, 5.9 and 7.4 did not exhibit any oxidative stress, one of the first and crucial events causing cell death “Are oxidative stress and mitochondrial dysfunction the key players in the neurodegenerative diseases?” (M. Di Carlo, D. Giacomazza, P. Picone, D. Nuzzo, P.L. San Biagio, 2012) [2] “Effect of zinc oxide nanomaterials induced oxidative stress on the p53 pathway” (M.I. Setyawati, C.Y. Tay, D.T. Leaong, 2013) [3].

【 授权许可】

Unknown